Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Nakagawa, H.; Arao, T.; Matsuzawa, T.; Ito, S.; Ogura, N.; Takehana, H.
    Comparison of the properties of tomato beta-fructofuranosidase embedded within a polyacrylamide gel and adsorbed on CM-cellulose (1975), Agric. Biol. Chem., 39, 1-5.
No PubMed abstract available

General Stability

EC Number General Stability Organism
3.2.1.26 enzyme embedded within a polyacrylamide gel and freeze dried retains only 25% of their original activity Solanum lycopersicum

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.26 Ag+ 0.1 mM, 95% inhibition of the CM-cellulose adsorbed enzyme, enzyme embedded within a polyacrylamide gel is inhibited 72.7% Solanum lycopersicum
3.2.1.26 Cu2+ CuSO4 Solanum lycopersicum
3.2.1.26 PCMB 0.1 mM, 94% inhibition of the CM-cellulose adsorbed enzyme, enzyme embedded within a polyacrylamide gel is inhibited 16.7% Solanum lycopersicum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.26 1.4
-
 sucrose enzyme embedded within a polyacrylamide gel Solanum lycopersicum
3.2.1.26 6.9
-
 sucrose enzyme adsorbed on CM-cellulose Solanum lycopersicum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.26 K4Fe(CN)6 activates enzyme embedded within a polyacrylamide gel, no effect on CM-cellulose-bound enzyme Solanum lycopersicum

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.26 Solanum lycopersicum
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.26 sucrose + H2O
-
 Solanum lycopersicum D-fructose + D-glucose
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.26 65
-
 5 min, complete inactivation of the CM-cellulose adsorbed enzyme Solanum lycopersicum
3.2.1.26 70
-
 5 min, enzyme embedded within a polyacrylamide gel loses 85% loss of activity Solanum lycopersicum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.26 4.5
-
 free enzyme and enzyme embedded within a polyacrylamide gel Solanum lycopersicum

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.26 3.3 6 pH 3.3: about 50% of maximal activity of the free enzyme, about 70% of maximal activity of the enzyme embedded within a polyacrylamide gel, pH 6.0: about 10% of activity of the free enzyme, about 45% of activity of the enzyme embedded within a polyacrylamide gel Solanum lycopersicum