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Literature summary extracted from

  • Nishizawa, M.; Maruyama, Y.; Nakamura, M.
    Purification and characterization of invertase isoenzymes from Fusarium oxysporum (1980), Agric. Biol. Chem., 44, 489-498.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.26 Ag+
-
 Fusarium oxysporum
3.2.1.26 Hg2+
-
 Fusarium oxysporum
3.2.1.26 PCMB
-
 Fusarium oxysporum
3.2.1.26 SDS
-
 Fusarium oxysporum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.26 2.1
-
 sucrose invertase P-2 Fusarium oxysporum
3.2.1.26 4.4
-
 sucrose invertase P-1 Fusarium oxysporum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.26 84000
-
 x * 84000, isoenzyme P-2, SDS-PAGE Fusarium oxysporum
3.2.1.26 108000
-
 x * 108000, isoenzyme P-1, SDS-PAGE Fusarium oxysporum
3.2.1.26 158000
-
 4°C, isoenzyme P-2, gel filtration Fusarium oxysporum
3.2.1.26 224000
-
 25°C, isoenzyme P-1, gel filtration Fusarium oxysporum
3.2.1.26 266000
-
 4°C, isoenzyme P-2, gel filtration Fusarium oxysporum
3.2.1.26 295000
-
 4°C, isonenzyme P-1, gel filtration Fusarium oxysporum

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.26 Fusarium oxysporum
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.26 glycoprotein isoenzyme P-1 contains 36% carbohydrate, isoenzyme P-2 contains 23% carbohydrate. Predominantly mannose with a smaller percentage of glucose, galactose, N-acetylglucosamine and N-acetylgalactosamine Fusarium oxysporum

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.26
-
 Fusarium oxysporum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.26 conidium isoenzyme P-2 Fusarium oxysporum
-
3.2.1.26 mycelium isoenzyme P-1 Fusarium oxysporum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.26 additional information
-
-
 Fusarium oxysporum
3.2.1.26 420
-
 isoenzyme P-1 Fusarium oxysporum
3.2.1.26 455
-
 isoenzyme P-2 Fusarium oxysporum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.26 raffinose + H2O
-
 Fusarium oxysporum fructose + melibiose
-
 ?
3.2.1.26 sucrose + H2O
-
 Fusarium oxysporum D-fructose + D-glucose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.26 ? x * 84000, isoenzyme P-2, SDS-PAGE Fusarium oxysporum
3.2.1.26 ? x * 108000, isoenzyme P-1, SDS-PAGE Fusarium oxysporum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.26 47
-
 invertase P-1 Fusarium oxysporum
3.2.1.26 52
-
 invertase P-2 Fusarium oxysporum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.26 55
-
 10 min, complete loss of activity of isoenzyme P-1, 20% loss of activity of isoenzyme P-2 Fusarium oxysporum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.26 4.5
-
 invertase P-1 Fusarium oxysporum
3.2.1.26 5
-
 invertase P-2 Fusarium oxysporum