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Literature summary extracted from
- Invertases of lilium pollen. Characterization and activity during in vitro germination (1984), Plant Physiol., 74, 510-515.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.2.1.26 | Cys | 1-10 mM, activates | Lilium auratum |  |
| 3.2.1.26 | reduced glutathione | activates | Lilium auratum | |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.2.1.26 | fructose 6-phosphate | slight inhibition | Lilium auratum | |
| 3.2.1.26 | PCMB | - |
Lilium auratum | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.2.1.26 | 0.65 | - |
sucrose | invertase 1 | Lilium auratum | |
| 3.2.1.26 | 0.98 | - |
sucrose | invertase 2 | Lilium auratum | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.2.1.26 | cell wall | invertase 2, enzyme can not released by treatment with detergents or high salt concentrations | Lilium auratum | 5618 | - |
| 3.2.1.26 | cytoplasm | invertase 1 | Lilium auratum | 5737 | - |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.2.1.26 | Co2+ | activates | Lilium auratum | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.26 | 450000 | - |
gel filtration | Lilium auratum |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.26 | Lilium auratum | - |
- |
- |
Posttranslational Modification
| EC Number | Posttranslational Modification | Comment | Organism |
|---|---|---|---|
| 3.2.1.26 | glycoprotein | invertase 1 is a glycoprotein | Lilium auratum |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.2.1.26 | invertase 1, partial | Lilium auratum |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.2.1.26 | pollen | - |
Lilium auratum | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.26 | 2970 | - |
- |
Lilium auratum |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.26 | melizitose + H2O | no activity with melizitose | Lilium auratum | ? | - |
? | |
| 3.2.1.26 | raffinose + H2O | 5.0% of the activity with sucrose | Lilium auratum | fructose + melibiose | - |
? | |
| 3.2.1.26 | stachyose + H2O | 2.6% of the activity with sucrose | Lilium auratum | ? | - |
? | |
| 3.2.1.26 | sucrose + H2O | - |
Lilium auratum | D-fructose + D-glucose | - |
? | |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.26 | 50 | - |
20 min, 60% loss of invertase 1 activity, 8% loss of invertase 2 activity | Lilium auratum |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.26 | 4 | - |
invertase 1 | Lilium auratum |
| 3.2.1.26 | 5.6 | - |
invertase 2 | Lilium auratum |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.26 | 3 | 5.4 | about 65% of maximal activity at pH 3.0 and at pH 5.4, cytoplasmic enzyme | Lilium auratum |
| 3.2.1.26 | 4.8 | 7.4 | pH 4.8: about 55% of maximal activity, pH 7.4: about 40% of maximal activity, pollen wall enzyme | Lilium auratum |
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