EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
3.2.1.26 | bovine serum albumin | stimulates intracellular enzyme | Saccharomyces cerevisiae |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.2.1.26 | 2-Amino-2-hydroxymethyl propane-1,3-diol | extracellular enzyme | Saccharomyces cerevisiae | |
3.2.1.26 | ammonium sulfate | intracellular enzyme | Saccharomyces cerevisiae | |
3.2.1.26 | aniline | extracellular enzyme | Saccharomyces cerevisiae | |
3.2.1.26 | I2 | extracellular enzyme | Saccharomyces cerevisiae | |
3.2.1.26 | NaCl | 0.1 M, partial, intracellular enzyme | Saccharomyces cerevisiae | |
3.2.1.26 | Sodium sulfate | 0.1 M, partial, intracellular enzyme | Saccharomyces cerevisiae |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.26 | 25 | 26 | sucrose | extracellular enzyme | Saccharomyces cerevisiae | |
3.2.1.26 | 150 | - |
raffinose | exteracellular enzyme | Saccharomyces cerevisiae |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.2.1.26 | Saccharomyces cerevisiae | - |
extracellular invertase and intracellular invertase | - |
EC Number | Posttranslational Modification | Comment | Organism |
---|---|---|---|
3.2.1.26 | glycoprotein | extracellular enzyme contains up to 50% carbohydrate | Saccharomyces cerevisiae |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.2.1.26 | intracellular and extracellular invertase | Saccharomyces cerevisiae |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.2.1.26 | 4700 | - |
intracellular enzyme | Saccharomyces cerevisiae |
3.2.1.26 | 4800 | - |
extracellular enzyme | Saccharomyces cerevisiae |
EC Number | Storage Stability | Organism |
---|---|---|
3.2.1.26 | below 0°C, lyophilized enzyme, stable for at least 1 year, extracellular enzyme | Saccharomyces cerevisiae |
3.2.1.26 | frozen in solution, pH 4.5-5.0, stable for a few months, extracellular enzyme | Saccharomyces cerevisiae |
3.2.1.26 | frozen, pH 7.0-7.5, stable for a few months | Saccharomyces cerevisiae |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.26 | methyl-beta-D-fructofuranoside + H2O | - |
Saccharomyces cerevisiae | ? | - |
? | |
3.2.1.26 | additional information | at high substrate concentrations, 1 M, transferase activity, transferring the beta-D-fructofuranosyl residue to primary alcohols such as methanol, ethanol and n-propanol, extracellular enzyme | Saccharomyces cerevisiae | ? | - |
? | |
3.2.1.26 | raffinose + H2O | - |
Saccharomyces cerevisiae | fructose + melibiose | - |
? | |
3.2.1.26 | sucrose + H2O | - |
Saccharomyces cerevisiae | D-fructose + D-glucose | beta-D-fructofuranose | ? |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.26 | 30 | - |
pH 3-7, 2 h, extracellular enzyme, stable | Saccharomyces cerevisiae |
3.2.1.26 | 56 | - |
15 min stable at pH 4.9, very unstable above pH 6, extracellular enzyme. Intracellular enzyme is unstable above pH 6, stable at pH 4.9 for 10 min | Saccharomyces cerevisiae |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.26 | 3.5 | 5 | extracellular and intracellular enzyme | Saccharomyces cerevisiae |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.26 | 3 | 7 | 30°C, 2 h, extracellular enzyme, stable | Saccharomyces cerevisiae |
3.2.1.26 | 6.5 | 9 | 30°C, 2 h, intracellular enzyme, stable. In presence of bovine serum albumin stable down to pH 4.9 | Saccharomyces cerevisiae |