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Literature summary extracted from

  • Goldstein, A.; Lampen, J.O.
    beta-D-Fructofuranoside fructohydrolase from yeast (1975), Methods Enzymol., 42C, 504-511.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.26 bovine serum albumin stimulates intracellular enzyme Saccharomyces cerevisiae

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.26 2-Amino-2-hydroxymethyl propane-1,3-diol extracellular enzyme Saccharomyces cerevisiae
3.2.1.26 ammonium sulfate intracellular enzyme Saccharomyces cerevisiae
3.2.1.26 aniline extracellular enzyme Saccharomyces cerevisiae
3.2.1.26 I2 extracellular enzyme Saccharomyces cerevisiae
3.2.1.26 NaCl 0.1 M, partial, intracellular enzyme Saccharomyces cerevisiae
3.2.1.26 Sodium sulfate 0.1 M, partial, intracellular enzyme Saccharomyces cerevisiae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.26 25 26 sucrose extracellular enzyme Saccharomyces cerevisiae
3.2.1.26 150
-
 raffinose exteracellular enzyme Saccharomyces cerevisiae

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.26 Saccharomyces cerevisiae
-
 extracellular invertase and intracellular invertase
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.26 glycoprotein extracellular enzyme contains up to 50% carbohydrate Saccharomyces cerevisiae

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.26 intracellular and extracellular invertase Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.26 4700
-
 intracellular enzyme Saccharomyces cerevisiae
3.2.1.26 4800
-
 extracellular enzyme Saccharomyces cerevisiae

Storage Stability

EC Number Storage Stability Organism
3.2.1.26 below 0°C, lyophilized enzyme, stable for at least 1 year, extracellular enzyme Saccharomyces cerevisiae
3.2.1.26 frozen in solution, pH 4.5-5.0, stable for a few months, extracellular enzyme Saccharomyces cerevisiae
3.2.1.26 frozen, pH 7.0-7.5, stable for a few months Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.26 methyl-beta-D-fructofuranoside + H2O
-
 Saccharomyces cerevisiae ?
-
 ?
3.2.1.26 additional information at high substrate concentrations, 1 M, transferase activity, transferring the beta-D-fructofuranosyl residue to primary alcohols such as methanol, ethanol and n-propanol, extracellular enzyme Saccharomyces cerevisiae ?
-
 ?
3.2.1.26 raffinose + H2O
-
 Saccharomyces cerevisiae fructose + melibiose
-
 ?
3.2.1.26 sucrose + H2O
-
 Saccharomyces cerevisiae D-fructose + D-glucose beta-D-fructofuranose ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.26 30
-
 pH 3-7, 2 h, extracellular enzyme, stable Saccharomyces cerevisiae
3.2.1.26 56
-
 15 min stable at pH 4.9, very unstable above pH 6, extracellular enzyme. Intracellular enzyme is unstable above pH 6, stable at pH 4.9 for 10 min Saccharomyces cerevisiae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.26 3.5 5 extracellular and intracellular enzyme Saccharomyces cerevisiae

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.26 3 7 30°C, 2 h, extracellular enzyme, stable Saccharomyces cerevisiae
3.2.1.26 6.5 9 30°C, 2 h, intracellular enzyme, stable. In presence of bovine serum albumin stable down to pH 4.9 Saccharomyces cerevisiae