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Literature summary extracted from

  • Duffaud, G.D.; McCutchen, C.M.; Leduc, P.; Parker, K.N.; Kelly, R.M.
    Purification and characterisation of extremely thermostable beta-mannanase, beta-mannosidase, and alpha-galactosidase from the hyperthermophilic eubacterium Thermotoga neapolitana 5068 (1997), Appl. Environ. Microbiol., 63, 169-177.
    View publication on PubMedView publication on EuropePMC

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.25 200000
-
 gel filtration Thermotoga neapolitana
3.2.1.78 65000
-
 SDS-PAGE Thermotoga neapolitana

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.25 Thermotoga neapolitana
-
 5068
-
3.2.1.25 Thermotoga neapolitana 5068
-
 5068
-
3.2.1.78 Thermotoga neapolitana
-
 5068
-
3.2.1.78 Thermotoga neapolitana 5068
-
 5068
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.25
-
 Thermotoga neapolitana
3.2.1.78
-
 Thermotoga neapolitana

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.78 culture filtrate
-
 Thermotoga neapolitana
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.25 p-nitrophenyl-beta-D-mannoside + H2O
-
 Thermotoga neapolitana p-nitrophenol + alpha-D-mannose
-
 ?
3.2.1.25 p-nitrophenyl-beta-D-mannoside + H2O
-
 Thermotoga neapolitana 5068 p-nitrophenol + alpha-D-mannose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.25 ? x * 200000-220000, SDS-PAGE Thermotoga neapolitana

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.25 87
-
-
 Thermotoga neapolitana
3.2.1.78 90 92
-
 Thermotoga neapolitana

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.25 70 90 70°C: about 40% of maximal activity, 90°C: about 80% of maximal activity Thermotoga neapolitana

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.25 85
-
 half-life: 18 h Thermotoga neapolitana
3.2.1.25 90
-
 half-life: 42 min Thermotoga neapolitana
3.2.1.25 98
-
 half-life: 2 min Thermotoga neapolitana
3.2.1.78 91
-
 half-life 13 h Thermotoga neapolitana

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.25 7.7
-
-
 Thermotoga neapolitana
3.2.1.78 6.9
-
-
 Thermotoga neapolitana

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.25 6.5 9 pH 6.5: about 35% of maximal activity, pH 9.0: about 40% of maximal activity Thermotoga neapolitana