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Literature summary extracted from

  • Gubitz, G.M.; Hayn, M.; Sommerauer, M.; Steiner, W.
    Mannan-degrading enzymes from Sclerotium rolfsii: characterisation and synergism of two endo beta-mannanases and a beta-mannosidase (1996), Bioresour. Technol., 58, 127-135.
No PubMed abstract available

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.25 57500
-
 x * 57500, SDS-PAGE Athelia rolfsii
3.2.1.78 41900
-
-
 Athelia rolfsii
3.2.1.78 61200
-
-
 Athelia rolfsii

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.25 Athelia rolfsii
-
-
-
3.2.1.78 Athelia rolfsii
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.25
-
 Athelia rolfsii

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.78 380
-
 41.9 kDa protein Athelia rolfsii
3.2.1.78 475
-
 61.2 kDa protein Athelia rolfsii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.25 mannan + H2O glucomannans and galactomannans are completely hydrolyzed Athelia rolfsii mannose + ?
-
 ?
3.2.1.78 galactoglucomannan + H2O
-
 Athelia rolfsii ?
-
 ?
3.2.1.78 galactomannan + H2O
-
 Athelia rolfsii hydrolyzed galactomannan
-
 ?
3.2.1.78 glucomannan + H2O
-
 Athelia rolfsii oligosaccharides
-
 ?
3.2.1.78 mannan + H2O
-
 Athelia rolfsii oligosaccharides
-
 ?
3.2.1.78 additional information
-
 Athelia rolfsii ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.25 ? x * 57500, SDS-PAGE Athelia rolfsii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.25 55
-
-
 Athelia rolfsii
3.2.1.78 72
-
 41.9 kDa protein Athelia rolfsii
3.2.1.78 74
-
 61.2 kDa protein Athelia rolfsii

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.25 50
-
 pH 4.5, half-life: 710 min Athelia rolfsii
3.2.1.25 65
-
 1 h, pH 4.5, about 50% loss of activity Athelia rolfsii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.25 2.5
-
-
 Athelia rolfsii
3.2.1.78 2.9
-
 61.2 kDa protein Athelia rolfsii
3.2.1.78 3.3
-
 41.9 kDa protein Athelia rolfsii

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.25 4.5
-
 most stable at Athelia rolfsii
3.2.1.78 4 5
-
 Athelia rolfsii