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Literature summary extracted from

  • Guadalupi, R.; Bernard, M.; Orlacchio, A.; Foglietti, M.J.; Emiliani, C.
    Purification and properties of human urinary beta-D-mannosidase (1996), Biochim. Biophys. Acta, 1293, 9-16.
    View publication on PubMed

General Stability

EC Number General Stability Organism
3.2.1.25 both forms of enzyme are partially stabilized by human albumin Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.25 0.13
-
 4-methylumbelliferyl beta-D-mannopyranoside beta-mannosidase B Homo sapiens
3.2.1.25 0.35
-
 4-methylumbelliferyl beta-D-mannopyranoside beta-mannosidase A Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.25 lysosome
-
 Homo sapiens 5764
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.25 37000
-
 1 * 37000 + 1 * 49000 + 1 * 75000, beta-mannosidase A, SDS-PAGE Homo sapiens
3.2.1.25 49000
-
 1 * 37000 + 1 * 49000 + 1 * 75000, beta-mannosidase A, SDS-PAGE Homo sapiens
3.2.1.25 49000
-
 beta-mannosidase B, 2 * 49000 + 1 * 75000, SDS-PAGE Homo sapiens
3.2.1.25 75000
-
 1 * 37000 + 1 * 49000 + 1 * 75000, beta-mannosidase A, SDS-PAGE Homo sapiens
3.2.1.25 75000
-
 beta-mannosidase B, 2 * 49000 + 1 * 75000, SDS-PAGE Homo sapiens
3.2.1.25 135000
-
 beta-mannosidase A, enzyme from urine, nondenaturing gradient PAGE Homo sapiens
3.2.1.25 137000
-
 beta-mannosidase A, enzyme from kidney, gradient gel electrophoresis Homo sapiens
3.2.1.25 155000
-
 beta-mannosidase B, enzyme from kidney, gradient gel electrophoresis Homo sapiens
3.2.1.25 160000
-
 beta-mannosidase B, enzyme from urine, nondenaturing gradient PAGE Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.25 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.25 two forms of enzyme: beta-mannosidase A and B Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.25 urine
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.25 1.772
-
-
 Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.25 4-methylumbelliferyl beta-D-mannoside + H2O
-
 Homo sapiens 4-methylumbelliferone + beta-D-mannose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.25 trimer 1 * 37000 + 1 * 49000 + 1 * 75000, beta-mannosidase A, SDS-PAGE Homo sapiens
3.2.1.25 trimer beta-mannosidase B, 2 * 49000 + 1 * 75000, SDS-PAGE Homo sapiens

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.25 37
-
 1 h, stable Homo sapiens
3.2.1.25 40
-
 1 h, stable Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.25 4.3
-
-
 Homo sapiens