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Literature summary extracted from

  • Houston, C.W.; Latimer, S.B.; Mitchell, E.D.
    Purification and properties of beta-mannosidase from malted barley (1974), Biochim. Biophys. Acta, 370, 276-282.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.25 p-nitrophenyl-alpha-D-mannopyranoside activates at low concentration, competitively inhibits at higher concentrations, 2-3 mM Hordeum vulgare

General Stability

EC Number General Stability Organism
3.2.1.25 no inactivation by freezing and thawing Hordeum vulgare

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.25 2-amino-2-deoxy-D-mannose competitive Hordeum vulgare
3.2.1.25 p-nitrophenyl-alpha-D-mannopyranoside activates at low concentration, competitively inhibits at higher concentrations, 2-3 mM Hordeum vulgare

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.25 0.32
-
 p-nitrophenyl-beta-D-mannoside
-
 Hordeum vulgare

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.25 88000
-
 x * 88000, SDS-PAGE Hordeum vulgare

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.25 Hordeum vulgare
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.25 glycoprotein 0.01% carbohydrate covalently bound to the partially purified enzyme Hordeum vulgare

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.25
-
 Hordeum vulgare

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.25 0.506
-
-
 Hordeum vulgare

Storage Stability

EC Number Storage Stability Organism
3.2.1.25 -10°C, lyophilized enzyme, stable for over a year Hordeum vulgare
3.2.1.25 4°C, in aqueous solution, stable for several months Hordeum vulgare

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.25 p-nitrophenyl-beta-D-mannoside + H2O p-nitrophenyl-beta-D-mannopyranoside Hordeum vulgare p-nitrophenol + alpha-D-mannose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.25 ? x * 88000, SDS-PAGE Hordeum vulgare

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.25 55
-
-
 Hordeum vulgare

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.25 5.5
-
-
 Hordeum vulgare