Literature summary extracted from

  • McCleary, B.V.
    exo-beta-D-Mannanase (1988), Methods Enzymol., 160, 589-595.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.25 Ag+
-
Cyamopsis tetragonoloba
3.2.1.25 Cu2+
-
Cyamopsis tetragonoloba
3.2.1.25 D-mannose 10 mM Cyamopsis tetragonoloba
3.2.1.25 Hg2+
-
Cyamopsis tetragonoloba
3.2.1.25 Zn2+
-
Cyamopsis tetragonoloba

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.25 0.5
-
p-nitrophenyl-beta-D-mannopyranoside
-
Cyamopsis tetragonoloba
3.2.1.25 0.5
-
4-methylumbelliferyl beta-D-mannopyranoside
-
Cyamopsis tetragonoloba
3.2.1.25 3
-
o-nitrophenyl-beta-D-mannopyranoside
-
Cyamopsis tetragonoloba
3.2.1.25 12
-
beta-D-mannotetraitol
-
Cyamopsis tetragonoloba
3.2.1.25 2.8
-
Beta-D-mannohexaitol
-
Cyamopsis tetragonoloba
3.2.1.25 2.8
-
beta-D-mannopentaitol
-
Cyamopsis tetragonoloba
3.2.1.25 80
-
beta-D-mannotriitol
-
Cyamopsis tetragonoloba

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.25 59000
-
gel filtration Cyamopsis tetragonoloba

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.25 additional information Cyamopsis tetragonoloba the enzyme plays a key role in the hydrolysis of galactomannan in germinating guar seed ?
-
?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.25 Cyamopsis tetragonoloba
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.25 glycoprotein contains 7% carbohydrate Cyamopsis tetragonoloba

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.25
-
Cyamopsis tetragonoloba

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.

Subunits

EC Number Subunits Comment Organism

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism