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Literature summary extracted from
- Glycosidases of Aspergillus niger. IV. Purification and characterization of alpha-mannosidase (1972), J. Biol. Chem., 247, 1780-1787.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.2.1.24 | Ag+ | - |
Aspergillus niger |  |
| 3.2.1.24 | Ba2+ | inhibition at 1 mM | Aspergillus niger | |
| 3.2.1.24 | Ca2+ | inhibition at 0.1 mM | Aspergillus niger | |
| 3.2.1.24 | Cd2+ | inhibition at 0.1 mM | Aspergillus niger | |
| 3.2.1.24 | Cu2+ | inhibition at 0.1 mM | Aspergillus niger | |
| 3.2.1.24 | D-mannono-1,5-lactone | complete inhibition at 1 mM | Aspergillus niger | |
| 3.2.1.24 | Fe3+ | inhibition at 1 mM | Aspergillus niger | |
| 3.2.1.24 | Hg2+ | strong inhibitor | Aspergillus niger | |
| 3.2.1.24 | Mg2+ | inhibition at 1 mM | Aspergillus niger | |
| 3.2.1.24 | Mn2+ | inhibition at 1 mM | Aspergillus niger | |
| 3.2.1.24 | Sr2+ | inhibition at 1 mM | Aspergillus niger | |
| 3.2.1.24 | Zn2+ | inhibition at 1 mM | Aspergillus niger | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.2.1.24 | 1.2 | - |
p-nitrophenyl-alpha-D-mannopyranoside | - |
Aspergillus niger | |
| 3.2.1.24 | 8.2 | - |
methyl-4-O-alpha-D-mannopyranosyl-D-mannopyranoside | - |
Aspergillus niger | |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.24 | 2-O-alpha-D-mannopyranosyl-D-mannopyranose + H2O | Aspergillus niger | very low activity | 2 alpha-D-mannopyranose | - |
? | |
| 3.2.1.24 | 3-O-alpha-D-mannopyranosyl-D-mannopyranose + H2O | Aspergillus niger | very low activity | 2 alpha-D-mannopyranose | - |
? | |
| 3.2.1.24 | 4-O-alpha-D-mannopyranosyl-D-mannopyranose + H2O | Aspergillus niger | specific cleavage of alpha-1,4- or alpha-1,6-linkages | 2 alpha-D-mannopyranose | - |
? | |
| 3.2.1.24 | 6-O-alpha-D-mannopyranosyl-D-mannopyranose + H2O | Aspergillus niger | specific cleavage of alpha-1,4- or alpha-1,6-linkages | alpha-D-mannopyranose | - |
? | |
| 3.2.1.24 | glycopeptide + H2O | Aspergillus niger | faster hydrolysis than for glycoprotein | alpha-D-mannopyranose + ? | - |
? | |
| 3.2.1.24 | glycoprotein + H2O | Aspergillus niger | - |
alpha-D-mannopyranose + ? | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.24 | Aspergillus niger | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.2.1.24 | to homogeneity, chromatography steps | Aspergillus niger |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.2.1.24 | commercial preparation | Rhozyme HP-150, crude enzyme preparation | Aspergillus niger | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.24 | 2-acetamido-4-O-alpha-D-mannopyranosyl-2-deoxy-D-glucose + H2O | - |
Aspergillus niger | alpha-D-mannopyranose + ? | - |
? | |
| 3.2.1.24 | 2-O-alpha-D-mannopyranosyl-D-mannopyranose + H2O | very low activity | Aspergillus niger | alpha-D-mannopyranose + D-mannopyranose | - |
? | |
| 3.2.1.24 | 2-O-alpha-D-mannopyranosyl-D-mannopyranose + H2O | very low activity | Aspergillus niger | 2 alpha-D-mannopyranose | - |
? | |
| 3.2.1.24 | 3-O-alpha-D-mannopyranosyl-D-mannopyranose + H2O | very low activity | Aspergillus niger | 2 alpha-D-mannopyranose | - |
? | |
| 3.2.1.24 | 4-O-alpha-D-mannopyranosyl-D-mannopyranose + H2O | specific cleavage of alpha-1,4- or alpha-1,6-linkages | Aspergillus niger | 2 alpha-D-mannopyranose | - |
? | |
| 3.2.1.24 | 6-O-alpha-D-mannopyranosyl-D-mannopyranose + H2O | specific cleavage of alpha-1,4- or alpha-1,6-linkages | Aspergillus niger | alpha-D-mannopyranose | - |
? | |
| 3.2.1.24 | glycopeptide + H2O | faster hydrolysis than for glycoprotein | Aspergillus niger | alpha-D-mannopyranose + ? | - |
? | |
| 3.2.1.24 | glycoprotein + H2O | - |
Aspergillus niger | alpha-D-mannopyranose + ? | - |
? | |
| 3.2.1.24 | methyl-2-O-alpha-D-mannopyranosyl-D-mannopyranoside + H2O | very low activity | Aspergillus niger | alpha-D-mannopyranose + ? | - |
? | |
| 3.2.1.24 | methyl-4-O-alpha-D-mannopyranosyl-D-mannopyranoside + H2O | - |
Aspergillus niger | alpha-D-mannopyranose + ? | - |
? | |
| 3.2.1.24 | p-nitrophenyl-alpha-D-mannopyranoside + H2O | very low activity | Aspergillus niger | p-nitrophenol + alpha-D-mannopyranose | - |
? | |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.24 | 45 | - |
- |
Aspergillus niger |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.24 | 10 | 45 | stable within | Aspergillus niger |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.24 | 4.2 | - |
- |
Aspergillus niger |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.24 | 5 | 7 | stable within | Aspergillus niger |
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