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Literature summary extracted from

  • Augustin, J.; Sikl, D.; Zemek, J.
    Properties of extracellular alpha-mannosidase from Aspergillus flavus (1978), Collect. Czech. Chem. Commun., 43, 2812-2816.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.24 NaCl increasing activity up to 1.6 M Aspergillus flavus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.24 Cu2+ 87% inhibition at 20 mM Aspergillus flavus
3.2.1.24 Fe2+ 22% inhibition at 20 mM Aspergillus flavus
3.2.1.24 Li+ 88% inhibition at 20 mM Aspergillus flavus
3.2.1.24 Mg2+ 61% inhibition at 20 mM Aspergillus flavus
3.2.1.24 Zn2+ 25% inhibition at 20 mM Aspergillus flavus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.24 1.4
-
 p-nitrophenyl-alpha-D-mannopyranoside
-
 Aspergillus flavus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.24 extracellular
-
 Aspergillus flavus
-
-

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.24 Aspergillus flavus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.24 partial Aspergillus flavus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.24 cell culture
-
 Aspergillus flavus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.24 87.7
-
-
 Aspergillus flavus

Storage Stability

EC Number Storage Stability Organism
3.2.1.24 -10°C, several weeks Aspergillus flavus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.24 p-nitrophenyl-alpha-D-mannopyranoside + H2O
-
 Aspergillus flavus p-nitrophenol + alpha-D-mannopyranose
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.24 37 40
-
 Aspergillus flavus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.24 50
-
 complete loss of activity after 15 min Aspergillus flavus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.24 4.2 4.5
-
 Aspergillus flavus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.24 6.3 6.8 most stable within Aspergillus flavus