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Literature summary extracted from

  • Opheim, D.J.; Touster, O.
    alpha-D-Mannosidase from rat liver lysosomes (1978), Methods Enzymol., 50, 494-500.
    View publication on PubMed

General Stability

EC Number General Stability Organism
3.2.1.24 albumin protects activity Rattus sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.24 alpha-D-mannopyranose competitive inhibition, Ki: 85 mM Rattus sp.
3.2.1.24 D-mannono-1,4-lactone Ki: 23.8 mM Rattus sp.
3.2.1.24 EDTA addition of Zn2+ restores inhibitory effect Rattus sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.24 10
-
 p-nitrophenyl-alpha-D-mannopyranoside
-
 Rattus sp.

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.24 lysosome
-
 Rattus sp. 5764
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.24 335000
-
 gel filtration Rattus sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.24 beta-glucuronidase peptide + H2O Rattus sp.
-
 alpha-D-mannopyranose + ?
-
 ?
3.2.1.24 GDP-mannosylpyranose + H2O Rattus sp.
-
 alpha-D-mannopyranose + GDP
-
 ?
3.2.1.24 glycopeptide + H2O Rattus sp. from ovalbumin alpha-D-mannopyranose + ?
-
 ?
3.2.1.24 mannopyranosyl-oligosaccharides + H2O Rattus sp. from yeast mannan, hydrolysis of alpha-1,2- and alpha-1,3-linkages alpha-D-mannopyranose + ?
-
 ?
3.2.1.24 thyroglobulin peptide A + H2O Rattus sp.
-
 alpha-D-mannopyranose + ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.24 Rattus sp.
-
-
-

Oxidation Stability

EC Number Oxidation Stability Organism
3.2.1.24 2-mercaptoethanol required during purification Rattus sp.

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.24 glycoprotein
-
 Rattus sp.

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.24 partial Rattus sp.

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.24 liver lysosomal type Rattus sp.
-

Storage Stability

EC Number Storage Stability Organism
3.2.1.24 4°C, 10 mM sodium acetate, pH 6.0, high protein concentration, 1 month Rattus sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.24 beta-glucuronidase peptide + H2O
-
 Rattus sp. alpha-D-mannopyranose + ?
-
 ?
3.2.1.24 GDP-mannosylpyranose + H2O
-
 Rattus sp. alpha-D-mannosylpyranose + GDP
-
 ?
3.2.1.24 GDP-mannosylpyranose + H2O
-
 Rattus sp. alpha-D-mannopyranose + GDP
-
 ?
3.2.1.24 glycopeptide + H2O from ovalbumin Rattus sp. alpha-D-mannopyranose + ?
-
 ?
3.2.1.24 mannopyranosyl-oligosaccharides + H2O from yeast mannan, hydrolysis of alpha-1,2- and alpha-1,3-linkages Rattus sp. alpha-D-mannopyranose + ?
-
 ?
3.2.1.24 methyl-alpha-D-mannopyranoside + H2O
-
 Rattus sp. alpha-D-mannopyranose + methanol
-
 ?
3.2.1.24 p-nitrophenyl-alpha-D-mannopyranoside + H2O
-
 Rattus sp. p-nitrophenol + alpha-D-mannopyranose
-
 ?
3.2.1.24 thyroglobulin peptide A + H2O
-
 Rattus sp. alpha-D-mannopyranose + ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.24 lysosomal alpha-D-mannosidase
-
 Rattus sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.24 40
-
 increasing activity up to Rattus sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.24 4.6
-
-
 Rattus sp.

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.24 3.8 5.5 50% activity at pH 3.8 and pH 5.5 Rattus sp.

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.2.1.24 23.8
-
 D-mannono- 1,4 -lactone
-
 Rattus sp.