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Literature summary extracted from
- alpha-D-Mannosidase and alpha-D-galactosidase from protein bodies of Lupinus angustifolius cotyledons (1982), Phytochemistry, 21, 985-989.
No PubMed abstract available
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.2.1.22 | Ag+ | - |
Lupinus angustifolius |  |
| 3.2.1.22 | D-galactose | - |
Lupinus angustifolius | |
| 3.2.1.22 | Hg2+ | - |
Lupinus angustifolius | |
| 3.2.1.24 | Ag+ | 97% inhibition at 10 mM | Lupinus angustifolius | |
| 3.2.1.24 | alpha-conglutin | 11% inhibition at saturation | Lupinus angustifolius | |
| 3.2.1.24 | alpha-D-mannopyranose | 29% inhibition at 10 mM | Lupinus angustifolius | |
| 3.2.1.24 | Cu2+ | 10% inhibition at 10 mM, alpha-mannosidase A | Lupinus angustifolius | |
| 3.2.1.24 | EDTA | addition of Zn2+ restores inhibitory effect | Lupinus angustifolius | |
| 3.2.1.24 | Hg2+ | 45% inhibition at 10 mM | Lupinus angustifolius | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.2.1.22 | 0.28 | - |
p-nitrophenyl alpha-D-galactose | alpha-galactosidase I | Lupinus angustifolius | |
| 3.2.1.22 | 0.56 | - |
p-nitrophenyl alpha-D-galactose | alpha-galactosidase II | Lupinus angustifolius | |
| 3.2.1.24 | 1.92 | - |
p-nitrophenyl-alpha-D-mannopyranoside | alpha-mannosidase A | Lupinus angustifolius | |
| 3.2.1.24 | 2.7 | - |
p-nitrophenyl-alpha-D-mannopyranoside | alpha-mannosidase B | Lupinus angustifolius | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.2.1.24 | protein storage vacuole | - |
Lupinus angustifolius | 326 | - |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.2.1.24 | Zn2+ | maximum activity at 1 mM | Lupinus angustifolius | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.22 | 17000 | - |
alpha-galactosidase II, gel filtration | Lupinus angustifolius |
| 3.2.1.22 | 70800 | - |
alpha-galactosidase I, gel filtration | Lupinus angustifolius |
| 3.2.1.24 | 300000 | - |
gel filtration, alpha-mannosidases A and B | Lupinus angustifolius |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.24 | alpha-conglutin + H2O | Lupinus angustifolius | lupin seed storage glycoprotein, not active to beta- or gamma-conglutin | alpha-D-mannopyranose + ? | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.22 | Lupinus angustifolius | - |
- |
- |
| 3.2.1.24 | Lupinus angustifolius | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.2.1.22 | - |
Lupinus angustifolius |
| 3.2.1.24 | to homogeneity, chromatography steps | Lupinus angustifolius |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.2.1.24 | cotyledon | alpha-mannosidases A and B | Lupinus angustifolius | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.22 | 285 | - |
alpha-galactosidase II | Lupinus angustifolius |
| 3.2.1.22 | 1600 | - |
alpha-galactosidase I | Lupinus angustifolius |
| 3.2.1.24 | 1.93 | - |
alpha-mannosidase B | Lupinus angustifolius |
| 3.2.1.24 | 15.94 | - |
alpha-mannosidase A | Lupinus angustifolius |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.22 | p-nitrophenyl-alpha-D-galactoside + H2O | - |
Lupinus angustifolius | p-nitrophenol + alpha-D-galactose | - |
? | |
| 3.2.1.24 | alpha-conglutin + H2O | lupin seed storage glycoprotein, not active to beta- or gamma-conglutin | Lupinus angustifolius | alpha-D-mannopyranose + ? | - |
? | |
| 3.2.1.24 | methyl-alpha-D-mannnopyranose + H2O | - |
Lupinus angustifolius | alpha-D-mannopyranose + methanol | - |
? | |
| 3.2.1.24 | p-nitrophenyl-alpha-D-mannopyranoside + H2O | - |
Lupinus angustifolius | p-nitrophenol + alpha-D-mannopyranose | - |
? | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.22 | 5.5 | - |
alpha-galactosidase II | Lupinus angustifolius |
| 3.2.1.22 | 6.5 | - |
alpha-galactosidase I | Lupinus angustifolius |
| 3.2.1.24 | 4 | - |
alpha-mannosidase A | Lupinus angustifolius |
| 3.2.1.24 | 4.6 | - |
alpha-mannosidase B | Lupinus angustifolius |
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