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Literature summary extracted from

  • Shigeta, S.; Kubota, H.; Tamura, H.
    Purification and characterization of an alpha-mannosidase of sea-squirt (1983), J. Biochem., 94, 1827-1832.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.24 Cu2+
-
 Styela plicata
3.2.1.24 diisopropylfluorophosphate 42% inhibition at 10 mM Styela plicata
3.2.1.24 EDTA addition of Zn2+ restores inhibitory effect Styela plicata
3.2.1.24 Fe2+
-
 Styela plicata
3.2.1.24 Hg2+
-
 Styela plicata

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.24 Zn2+ enhanced activity at 0.3-1 mM Styela plicata

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.24 70000
-
 4 * 70000, SDS-PAGE Styela plicata
3.2.1.24 275000
-
 gel filtration Styela plicata

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.24 glycopeptide + H2O Styela plicata from ovalbumin alpha-D-mannopyranose + ?
-
 ?
3.2.1.24 mannan + H2O Styela plicata from baker's and sake yeast alpha-D-mannose + ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.24 Styela plicata
-
 sea squirt
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.24 to homogeneity, chromatography steps Styela plicata

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.24 viscus
-
 Styela plicata
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.24 37
-
-
 Styela plicata

Storage Stability

EC Number Storage Stability Organism
3.2.1.24 -20°C, pH 6.0, 50% glycerol, 3 months Styela plicata

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.24 1-naphthyl-alpha-D-mannopyranoside + H2O
-
 Styela plicata 1-naphthol + alpha-D-mannopyranose
-
 ?
3.2.1.24 4-methylumbelliferyl-alpha-D-mannopyranoside + H2O
-
 Styela plicata 4-methylumbelliferone + alpha-D-mannopyranose
-
 ?
3.2.1.24 6-bromo-2-naphthyl-alpha-D-mannopyranoside + H2O
-
 Styela plicata 6-bromo-2-naphthol + alpha-D-mannopyranose
-
 ?
3.2.1.24 glycopeptide + H2O from ovalbumin Styela plicata alpha-D-mannopyranose + ?
-
 ?
3.2.1.24 mannan + H2O from baker's and sake yeast Styela plicata alpha-D-mannose + ?
-
 ?
3.2.1.24 p-nitrophenyl-alpha-D-mannopyranoside + H2O
-
 Styela plicata p-nitrophenol + alpha-D-mannopyranose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.24 tetramer 4 * 70000, SDS-PAGE Styela plicata

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.24 3.4
-
-
 Styela plicata

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.24 4 6
-
 Styela plicata