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Literature summary extracted from

  • Tulsiani, D.R.P.; Touster, O.
    Characterization of a novel alpha-D-mannosidase from rat brain microsomes (1985), J. Biol. Chem., 260, 13081-13087.
    View publication on PubMed

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.24 microsome
-
 Rattus sp.
-
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.24 alpha-D-Man-(1-2)-alpha-D-Man-(1-2)-alpha-D-Man-(1-3)-[alpha-D-Man-(1-6)-[alpha-D-Man-(1-2)-alpha-D-Man-(1-3)]-alpha-D-Man-(1-6)]-beta-D-Man-(1-4)-beta-D-GlcNAc + H2O Rattus sp. cleaves asparagine-linked high mannose oligosaccharides to form the core Man3GlcNAc2 alpha-D-Man-(1-6)-[alpha-D-Man-(1-3)]-beta-D-Man-(1-4)-beta-D-GlcNAc + alpha-D-Man-(1-3)-[alpha-D-Man-(1-6)]-alpha-D-Man-(1-6)-beta-D-Man
-
 ?
3.2.1.24 Man5GlcNAc + 2 H2O Rattus sp.
-
 Man3GlcNAc + 2 alpha-D-mannopyranose
-
 ?
3.2.1.24 Man5GlcNAc2 + 2 H2O Rattus sp.
-
 Man3GlcNAc2 + 2 alpha-D-mannopyranose
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.24 Rattus sp.
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.24 near homogeneitiy Rattus sp.

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.24 brain
-
 Rattus sp.
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.24 alpha-D-Man-(1-2)-alpha-D-Man-(1-2)-alpha-D-Man-(1-3)-[alpha-D-Man-(1-6)-[alpha-D-Man-(1-2)-alpha-D-Man-(1-3)]-alpha-D-Man-(1-6)]-beta-D-Man-(1-4)-beta-D-GlcNAc + H2O cleaves asparagine-linked high mannose oligosaccharides to form the core Man3GlcNAc2 Rattus sp. alpha-D-Man-(1-6)-[alpha-D-Man-(1-3)]-beta-D-Man-(1-4)-beta-D-GlcNAc + alpha-D-Man-(1-3)-[alpha-D-Man-(1-6)]-alpha-D-Man-(1-6)-beta-D-Man
-
 ?
3.2.1.24 Man5GlcNAc + 2 H2O
-
 Rattus sp. Man3GlcNAc + 2 alpha-D-mannopyranose
-
 ?
3.2.1.24 Man5GlcNAc2 + 2 H2O
-
 Rattus sp. Man3GlcNAc2 + 2 alpha-D-mannopyranose
-
 ?
3.2.1.24 p-nitrophenyl-alpha-D-mannopyranoside + H2O very poor substrate Rattus sp. p-nitrophenol + alpha-D-mannopyranose
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.24 6
-
-
 Rattus sp.

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.24 6
-
 90% activity after 60 min at 37°C Rattus sp.