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Literature summary extracted from

  • Nakagawa, H.; Enomoto, N.; Asakawa, M.; Uda, Y.
    Purification and characterization of alpha-mannosidase and beta-N-acetylhexosaminidase from watermelon fruit (1988), Agric. Biol. Chem., 52, 2223-2230.
No PubMed abstract available

General Stability

EC Number General Stability Organism
3.2.1.24 albumin protects activity Citrullus lanatus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.24 EDTA addition of Zn2+ restores inhibitory effect Citrullus lanatus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.24 2.3
-
 p-nitrophenyl-alpha-D-mannopyranoside
-
 Citrullus lanatus
3.2.1.52 0.65
-
 p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside
-
 Citrullus lanatus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.24 270000
-
 gel filtration Citrullus lanatus
3.2.1.52 150000
-
 gel filtration Citrullus lanatus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.24 glycopeptide + H2O Citrullus lanatus from ovalbumin alpha-D-mannopyranose + ?
-
 ?
3.2.1.52 N-acetyl-beta-D-hexosaminides + H2O Citrullus lanatus
-
 N-acetyl-D-hexosamines
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.24 Citrullus lanatus
-
-
-
3.2.1.52 Citrullus lanatus
-
 watermelon
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.24 to homogeneity, chromatography steps Citrullus lanatus
3.2.1.52 to homogeneity Citrullus lanatus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.24 fruit
-
 Citrullus lanatus
-
3.2.1.52 fruit
-
 Citrullus lanatus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.24 40
-
-
 Citrullus lanatus
3.2.1.52 92
-
-
 Citrullus lanatus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.24 glycopeptide + H2O from ovalbumin Citrullus lanatus alpha-D-mannopyranose + ?
-
 ?
3.2.1.24 p-nitrophenyl-alpha-D-mannopyranoside + H2O
-
 Citrullus lanatus p-nitrophenol + alpha-D-mannopyranose
-
 ?
3.2.1.52 N-acetyl-beta-D-hexosaminides + H2O
-
 Citrullus lanatus N-acetyl-D-hexosamines
-
 ?
3.2.1.52 p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside + H2O
-
 Citrullus lanatus 2-acetamido-2-deoxy-D-glucose + p-nitrophenol
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.24 4 4.5
-
 Citrullus lanatus
3.2.1.52 5 5.5 substrate: p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside Citrullus lanatus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.24 7 9 stable within Citrullus lanatus
3.2.1.52 6.5 8 fairly stable Citrullus lanatus