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Literature summary extracted from
- Alpha-D-mannosidase from the aleurone layers of resting wheat grains: sugar-depleted forms (1987), Phytochemistry, 26, 2909-2912.
No PubMed abstract available
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.2.1.24 | Co2+ | 57% inhibition at 1 mM | Triticum aestivum |  |
| 3.2.1.24 | EDTA | addition of Zn2+ restores inhibitory effect | Triticum aestivum | |
| 3.2.1.24 | swainsonine | competitive inhibition | Triticum aestivum | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.2.1.24 | Zn2+ | 25% stimulation | Triticum aestivum | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.24 | 188000 | 197000 | gel filtration, sucrose density gradient centrifugation | Triticum aestivum |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.24 | Triticum aestivum | - |
- |
- |
Posttranslational Modification
| EC Number | Posttranslational Modification | Comment | Organism |
|---|---|---|---|
| 3.2.1.24 | glycoprotein | sugar content, w/w: 20.1% glucose, 3.09% mannose, 1.7% galactose, less than 1%N-acetyl-D-glucosamine, minor component: sialic acid | Triticum aestivum |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.2.1.24 | to homogeneity, chromatography steps, affinity chromatography | Triticum aestivum |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.2.1.24 | aleurone layer | - |
Triticum aestivum | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.24 | 8.33 | - |
- |
Triticum aestivum |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.24 | p-nitrophenyl-alpha-D-mannopyranoside + H2O | - |
Triticum aestivum | p-nitrophenol + alpha-D-mannopyranose | - |
? | |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.24 | 48 | - |
- |
Triticum aestivum |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.24 | 35 | - |
half maximal activity | Triticum aestivum |
| 3.2.1.24 | 55 | - |
half maximal activity | Triticum aestivum |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.24 | 4.6 | 4.8 | - |
Triticum aestivum |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.24 | 5.6 | - |
half maximal activity | Triticum aestivum |
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Release 2023.1 (January 2023)
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