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Literature summary extracted from
- Glycoprotein biosynthesis in yeast: purification and characterization of the endoplasmatic reticulum Man9 processing alpha-mannosidase (1991), Glycobiology, 1, 605-614.
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 3.2.1.209 | presence of CHAPS necessary for maximum activity | Saccharomyces cerevisiae |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.2.1.209 | EDTA | - |
Saccharomyces cerevisiae |  |
| 3.2.1.209 | EGTA | complete inhibition at 5 mM | Saccharomyces cerevisiae | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.2.1.209 | 0.2 | - |
Man9GlcNAc | - |
Saccharomyces cerevisiae | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.2.1.209 | membrane | from microsomal membranes | Saccharomyces cerevisiae | 16020 | - |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.2.1.209 | Ca2+ | inhibition by EGTA can be 90% restored after addition of 0.1 mM | Saccharomyces cerevisiae | |
| 3.2.1.209 | Zn2+ | 30% stimulation of the EGTA-inhibited enzyme at 0.1 mM | Saccharomyces cerevisiae | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.209 | 23000 | - |
1 * 44000 + 1 * 23000, reducing SDS-PAGE | Saccharomyces cerevisiae |
| 3.2.1.209 | 44000 | - |
1 * 44000 + 1 * 23000, reducing SDS-PAGE | Saccharomyces cerevisiae |
| 3.2.1.209 | 60000 | 68000 | gel filtration | Saccharomyces cerevisiae |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.209 | Glc3Man9GlcNAc2 + H2O | Saccharomyces cerevisiae | from oligosaccharide-lipid | alpha-D-mannopyranose + ? | - |
? | |
| 3.2.1.209 | Man7GlcNAc2 + H2O | Saccharomyces cerevisiae | from oligosaccharide-lipid, very low activity | alpha-D-mannopyranose + ? | - |
? | |
| 3.2.1.209 | Man9GlcNAc2 + H2O | Saccharomyces cerevisiae | from thyroglobulin or oligosaccharide-lipid, trimming the substrate to Man8GlcNAc2, the substrate for oligosaccharide elongation | Man5GlcNAc2 + Man6GlcNAc2 + Man7GlcNAc2 + Man8GlcNAc2 + alpha-D-mannopyranose | only alpha-(1-2)-linked terminal mannoses on the alpha-3-branch of the Man9GlcNAc precursor dispensable | ? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.209 | Saccharomyces cerevisiae | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.2.1.209 | to homogeneity, chromatography steps | Saccharomyces cerevisiae |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.2.1.209 | cell culture | - |
Saccharomyces cerevisiae | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.209 | Glc3Man9GlcNAc2 + H2O | from oligosaccharide-lipid | Saccharomyces cerevisiae | alpha-D-mannopyranose + ? | - |
? | |
| 3.2.1.209 | Man7GlcNAc2 + H2O | from oligosaccharide-lipid, very low activity | Saccharomyces cerevisiae | alpha-D-mannopyranose + ? | - |
? | |
| 3.2.1.209 | Man9GlcNAc + H2O | - |
Saccharomyces cerevisiae | Man8GlcNAc + alpha-D-mannopyranose | - |
? | |
| 3.2.1.209 | Man9GlcNAc2 + H2O | from thyroglobulin or oligosaccharide-lipid, trimming the substrate to Man8GlcNAc2, the substrate for oligosaccharide elongation | Saccharomyces cerevisiae | Man5GlcNAc2 + Man6GlcNAc2 + Man7GlcNAc2 + Man8GlcNAc2 + alpha-D-mannopyranose | only alpha-(1-2)-linked terminal mannoses on the alpha-3-branch of the Man9GlcNAc precursor dispensable | ? | |
| 3.2.1.209 | p-nitrophenyl-alpha-D-mannopyranoside + H2O | - |
Saccharomyces cerevisiae | p-nitrophenol + alpha-D-mannopyranose | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.2.1.209 | dimer | 1 * 44000 + 1 * 23000, reducing SDS-PAGE | Saccharomyces cerevisiae |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.2.1.209 | Man9-alpha-mannosidase | - |
Saccharomyces cerevisiae |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.209 | 6.5 | - |
- |
Saccharomyces cerevisiae |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.209 | 6 | 6.8 | activity falls rapid below and above | Saccharomyces cerevisiae |
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