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Literature summary extracted from

  • Yamashiro, K.; Itoh, H.; Yamagishi, M.; Natsuka, S.; Mega, T.; Hase, S.
    Purification and characterization of neutral alpha-mannosidase from hen oviduct: studies on the activation mechanism of Co2+ (1997), J. Biochem., 122, 1174-1181.
    View publication on PubMed

General Stability

EC Number General Stability Organism
3.2.1.24 stable in the presence of Co2+ at 37°C Gallus gallus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.24 1-deoxymannojirimicin 19% inhibition at 0.01 mM Gallus gallus
3.2.1.24 Cu2+ complete inhibition of the Co2+ activated enzyme at 0.5 mM Gallus gallus
3.2.1.24 Pb2+ complete inhibition of the Co2+ activated enzyme at 0.5 mM Gallus gallus
3.2.1.24 swainsonine complete inhibition of the Co2+ activated enzyme at 0.01 mM Gallus gallus
3.2.1.24 Zn2+ complete inhibition of the Co2+ activated enzyme at 0.5 mM Gallus gallus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.24 0.44
-
 Manalpha(1-6)[Manalpha(1-3)]Manalpha(1-6)[Manalpha(1-)]Manbeta(1-4)GlcNAc
-
 Gallus gallus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.24 Co2+ 24fold activation after preincubation with 1 mM Gallus gallus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.24 110000
-
 4 * 110000, SDS-PAGE Gallus gallus
3.2.1.24 480000
-
 gel filtration Gallus gallus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.24 Man9GlcNAc + H2O Gallus gallus Co2+ activated alpha-mannosidase Manalpha(1-6)[Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)]Manbeta(1-4)GlcNAc + alpha-D-mannopyranose end products ?
3.2.1.24 Manalpha(1-6)[Manalpha(1-3)]Manalpha(1-6)[Manalpha(1-)]Manbeta(1-4)GlcNAc + H2O Gallus gallus 4 times faster hydrolysis than Manalpha(1-6)[Manalpha(1-3)]Manalpha(1-6)[Manalpha(1-3)]Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc alpha-D-mannopyranose + ?
-
 ?
3.2.1.24 Manalpha(1-6)[Manalpha(1-3)]Manalpha(1-6)[Manalpha(1-3)]Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O Gallus gallus
-
 alpha-D-mannopyranose + ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.24 Gallus gallus
-
 chicken
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.24 to homogeneity, chromatography steps Gallus gallus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.24 oviduct
-
 Gallus gallus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.24 11
-
-
 Gallus gallus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.24 Man9GlcNAc + H2O Co2+ activated alpha-mannosidase Gallus gallus Manalpha(1-6)[Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)]Manbeta(1-4)GlcNAc + alpha-D-mannopyranose end products ?
3.2.1.24 Manalpha(1-6)[Manalpha(1-3)]Manalpha(1-6)[Manalpha(1-)]Manbeta(1-4)GlcNAc + H2O 4 times faster hydrolysis than Manalpha(1-6)[Manalpha(1-3)]Manalpha(1-6)[Manalpha(1-3)]Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc Gallus gallus alpha-D-mannopyranose + ?
-
 ?
3.2.1.24 Manalpha(1-6)[Manalpha(1-3)]Manalpha(1-6)[Manalpha(1-3)]Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O
-
 Gallus gallus alpha-D-mannopyranose + ?
-
 ?
3.2.1.24 p-nitrophenyl-alpha-D-mannopyranoside + H2O
-
 Gallus gallus p-nitrophenol + alpha-D-mannopyranose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.24 tetramer 4 * 110000, SDS-PAGE Gallus gallus

Synonyms

EC Number Synonyms Comment Organism
3.2.1.24 neutral alpha-mannosidase
-
 Gallus gallus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.24 6.8
-
-
 Gallus gallus