Any feedback?
Literature summary extracted from
- Cloning, expression, purification, and characterization of the murine lysosomal acid alpha-mannosidase (1997), Biochim. Biophys. Acta, 1336, 132-146.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.2.1.24 | expression in Pichia pastoris | Mus musculus |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.2.1.24 | swainsonine | IC50: 0.00015 mM | Mus musculus |  |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.2.1.24 | 12.6 | - |
p-nitrophenyl-alpha-D-mannopyranoside | - |
Mus musculus | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.24 | 670000 | - |
gel filtration | Mus musculus |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.24 | alpha-D-mannosidosis fibroblasts + H2O | Mus musculus | - |
Manbeta(1-4)GlcNAc + ? | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.24 | Mus musculus | O09159 | - |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.2.1.24 | recombinant protein, to homogeneity | Mus musculus |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.2.1.24 | liver | cDNA library | Mus musculus | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.24 | 1.86 | - |
- |
Mus musculus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.24 | 6 pyridylamino-Man9GlcNAc2 + H2O | stepwise cleavage to Man2GlcNAc structures | Mus musculus | pyridylamino-Man8GlcNAc2 + pyridylamino-Man7GlcNAc2 + pyridylamino-Man6GlcNAc2 + pyridylamino-Man5GlcNAc2 + pyridylamino-Man4GlcNAc2 + pyridylamino-Man3GlcNAc2 + 21 alpha-D-mannopyranose | - |
? | |
| 3.2.1.24 | alpha-D-mannosidosis fibroblasts + H2O | - |
Mus musculus | Manbeta(1-4)GlcNAc + ? | - |
? | |
| 3.2.1.24 | p-nitrophenyl-alpha-D-mannopyranoside + H2O | - |
Mus musculus | p-nitrophenol + alpha-D-mannopyranose | - |
? | |
| 3.2.1.24 | pyridylamino-Man5GlcNAc + H2O | - |
Mus musculus | pyridylamino-Man4GlcNAc + pyridylamino-Man3-2GlcNAc + alpha-D-mannose | lesser degree of pyridylamino-Man3-2GlcNAc, stepwise cleavage to Man2GlcNAc structures | ? | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.24 | 4.5 | - |
- |
Mus musculus |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.24 | 4.3 | 6 | - |
Mus musculus |
IC50 Value
| EC Number | IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
|---|---|---|---|---|---|---|
| 3.2.1.24 | 0.00015 | - |
IC50: 0.00015 mM | Mus musculus | swainsonine | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
