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Literature summary extracted from
- Cloning, expression, purification, and characterization of the acid alpha-mannosidase from Trypanosoma cruzi (1998), Glycobiology, 8, 1183-1194.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.2.1.24 | expression in Pichia pastoris | Trypanosoma cruzi |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.2.1.24 | swainsonine | 50% inhibition at 0.00024 mM | Trypanosoma cruzi |  |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.2.1.24 | 4.3 | - |
p-nitrophenyl-alpha-D-mannopyranoside | - |
Trypanosoma cruzi | |
| 3.2.1.24 | 5.2 | - |
4-methylumbelliferyl-alpha-D-mannopyranoside | - |
Trypanosoma cruzi | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.24 | 359000 | - |
gel filtration | Trypanosoma cruzi |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.24 | Trypanosoma cruzi | - |
- |
- |
Posttranslational Modification
| EC Number | Posttranslational Modification | Comment | Organism |
|---|---|---|---|
| 3.2.1.24 | glycoprotein | presence of N-glycans | Trypanosoma cruzi |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.2.1.24 | recombinant protein, to homogeneity | Trypanosoma cruzi |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.2.1.24 | epimastigote | - |
Trypanosoma cruzi | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.24 | 0.72 | - |
- |
Trypanosoma cruzi |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.24 | 4-methylumbelliferyl-alpha-D-mannopyranoside + H2O | - |
Trypanosoma cruzi | 4-methylumbelliferone + alpha-D-mannopyranose | - |
? | |
| 3.2.1.24 | Manalpha(1-2)Manalpha(1-2)Manalpha(1-6)Manalpha(1-4)-anhydromannitol + H2O | - |
Trypanosoma cruzi | Manalpha(1-2)Manalpha(1-6)Manalpha(1-4)-anhydromannitol + Manalpha(1-6)Manalpha(1-4)-anhydromannitol + Manalpha(1-4)anhydromannitol + anhydromannitol + alpha-D-mannose | equal amounts, but only traces of Manalpha(1-4)-anhydromannitol | ? | |
| 3.2.1.24 | p-nitrophenyl-alpha-D-mannopyranoside + H2O | - |
Trypanosoma cruzi | p-nitrophenol + alpha-D-mannopyranose | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.2.1.24 | tetramer | heterotetramer composed of 57000 + 46000 subunits, SDS-PAGE, endo H deglycosylation | Trypanosoma cruzi |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.2.1.24 | acid alpha-mannosidase | - |
Trypanosoma cruzi |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.24 | 3.5 | - |
- |
Trypanosoma cruzi |
Ki Value [mM]
| EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.2.1.24 | 0.00024 | - |
swainsonine | - |
Trypanosoma cruzi | |
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