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Literature summary extracted from
- Purification and properties of major alpha-D-mannosidase in the luminal fluid of porcine epididymis (1999), Biochim. Biophys. Acta, 1432, 382-392.
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.2.1.24 | Golgi lumen | luminal fluid | Sus scrofa | 5796 | - |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.24 | 51000 | - |
alpha2, 2 * 63000, beta2, 2 * 51000, reducing SDS-PAGE | Sus scrofa |
| 3.2.1.24 | 63000 | - |
alpha2, 2 * 63000, beta2, 2 * 51000, reducing SDS-PAGE | Sus scrofa |
| 3.2.1.24 | 275000 | - |
gel filtration, non-reducing SDS-PAGE | Sus scrofa |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.24 | Sus scrofa | - |
lysosomal type of alpha-D-mannosidase | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.2.1.24 | to homogeneity, chromatography steps | Sus scrofa |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.2.1.24 | epididymis | cauda epididymis | Sus scrofa | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.24 | 48.5 | - |
- |
Sus scrofa |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.24 | p-nitrophenyl-alpha-D-mannopyranoside + H2O | high amounts of substrate hydrolysed at pH 3.0, at neutral pH very little substrate cleaved | Sus scrofa | p-nitrophenol + alpha-D-mannopyranose | - |
? |  |
| 3.2.1.24 | pyridylamino-Man9GlcNAc2 + H2O | high amounts of substrate hydrolysed at neutral pH, very little cleavage at pH 3.0 | Sus scrofa | pyridylamino-Man8GlcNAc2 + alpha-D-mannopyranose | hydrolysis products within 20 min | ? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.2.1.24 | tetramer | alpha2, 2 * 63000, beta2, 2 * 51000, reducing SDS-PAGE | Sus scrofa |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.24 | 4.6 | - |
- |
Sus scrofa |
| 3.2.1.24 | 7 | - |
- |
Sus scrofa |
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