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Literature summary extracted from

  • Ishii, S.; Yokotsuka, T.
    Purification and properties of endo-polygalacturonase from Aspergillus japonicus (1972), Agric. Biol. Chem., 36, 1885-1893.
No PubMed abstract available

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.15 35500
-
 gel filtration Aspergillus japonicus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.15 Aspergillus japonicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.15
-
 Aspergillus japonicus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.15 1362
-
-
 Aspergillus japonicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.15 additional information maceration of plant tissues Aspergillus japonicus ?
-
 ?
3.2.1.15 pectic acid + H2O
-
 Aspergillus japonicus galacturonic acid oligomers
-
 ?
3.2.1.15 pectin + H2O
-
 Aspergillus japonicus ?
-
 ?
3.2.1.15 polygalacturonate + H2O
-
 Aspergillus japonicus oligogalacturonates higher galacturonides progressively disappear with reaction time, and monogalacturonic acid, digalacturonic acid and trigalacturonic acids remain as the end products of the hydrolysis ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.15 50
-
 pH 4.5, stable below Aspergillus japonicus
3.2.1.15 65
-
 10 min, complete loss of activity Aspergillus japonicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.15 4.5
-
 hydrolysis of pectin or pectic acid Aspergillus japonicus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.15 4 5 4°C or 30°C, stable Aspergillus japonicus