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Literature summary extracted from

  • Urbanek, H.; Zalewska-Sobczak, J.A.
    Polygalacturonase of Botrytis cinerea E-200 Pers (1975), Biochim. Biophys. Acta, 377, 402-409.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.15 PCMB
-
 Botrytis cinerea
3.2.1.15 Tetranitromethane
-
 Botrytis cinerea
3.2.1.15 Urea
-
 Botrytis cinerea

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.15 Botrytis cinerea
-
 E-200 PERS
-
3.2.1.15 Botrytis cinerea E-200 PERS
-
 E-200 PERS
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.15
-
 Botrytis cinerea

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.15 culture medium
-
 Botrytis cinerea
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.15 2049
-
-
 Botrytis cinerea

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.15 alpha-D-galacturonosyl-(1,4)-O-alpha-D-galacturonate + H2O at 14% of the activity with polypectate Botrytis cinerea D-galacturonic acid
-
 ?
3.2.1.15 alpha-D-galacturonosyl-(1,4)-O-alpha-D-galacturonate + H2O at 14% of the activity with polypectate Botrytis cinerea E-200 PERS D-galacturonic acid
-
 ?
3.2.1.15 alpha-D-galacturonosyl-(1,4)-O-alpha-D-galacturonosyl-(1,4)-O-alpha-D-galacturonate + H2O 52% of the activity with polypectate Botrytis cinerea digalacturonic acid + galacturonate
-
 ?
3.2.1.15 alpha-D-galacturonosyl-(1,4)-O-alpha-D-galacturonosyl-(1,4)-O-alpha-D-galacturonate + H2O 52% of the activity with polypectate Botrytis cinerea E-200 PERS digalacturonic acid + galacturonate
-
 ?
3.2.1.15 alpha-D-galacturonosyl-(1,4)-O-alpha-D-galacturonosyl-(1,4)-O-alpha-D-galacturonosyl-(1,4)-O-alpha-D-galacturonate + H2O 79% of the activity with polypectate Botrytis cinerea alpha-D-galacturonosyl-(1,4)-O-alpha-D-galacturonosyl-(1,4)-O-alpha-D-galacturonate + D-galacturonate
-
 ?
3.2.1.15 alpha-D-galacturonosyl-(1,4)-O-alpha-D-galacturonosyl-(1,4)-O-alpha-D-galacturonosyl-(1,4)-O-alpha-D-galacturonate + H2O 79% of the activity with polypectate Botrytis cinerea E-200 PERS alpha-D-galacturonosyl-(1,4)-O-alpha-D-galacturonosyl-(1,4)-O-alpha-D-galacturonate + D-galacturonate
-
 ?
3.2.1.15 polygalacturonate + H2O
-
 Botrytis cinerea oligogalacturonates
-
 ?
3.2.1.15 polygalacturonate + H2O
-
 Botrytis cinerea E-200 PERS oligogalacturonates
-
 ?
3.2.1.15 polypectate + H2O oligogalacturonides gradually disappear with the reaction time, so that monogalacturonic acid remains as the only product of the hydrolysis Botrytis cinerea ?
-
 ?
3.2.1.15 polypectate + H2O oligogalacturonides gradually disappear with the reaction time, so that monogalacturonic acid remains as the only product of the hydrolysis Botrytis cinerea E-200 PERS ?
-
 ?