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Literature summary extracted from

  • Brandt, V.; Geerlings, A.; Tits, M.; Delaude, C.; van der Heijden, R.; Verpoorte, R.; Angenot, L.
    New strictosidine beta-glucosidase from Strychnos mellodora (2000), Plant Physiol. Biochem., 38, 187-192.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.105 D-gluconolactone strong inhibitor Catharanthus roseus
3.2.1.105 D-gluconolactone strong inhibitor Strychnos mellodora

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.105 0.01 0.018 strictosidine
-
Catharanthus roseus
3.2.1.105 0.12
-
strictosidine
-
Strychnos mellodora
3.2.1.105 0.12
-
strictosidine pH 6.3, 30°C Strychnos mellodora
3.2.1.105 0.335
-
palicoside
-
Strychnos mellodora
3.2.1.105 0.335
-
palicoside pH 6.3, 30°C Strychnos mellodora
3.2.1.105 0.564
-
dolichantoside
-
Strychnos mellodora
3.2.1.105 0.564
-
dolichantoside pH 6.3, 30°C Strychnos mellodora
3.2.1.105 1.13
-
dolichantoside
-
Catharanthus roseus
3.2.1.105 1.13
-
dolichantoside pH 6.3, 30°C Catharanthus roseus
3.2.1.105 7.015
-
palicoside
-
Catharanthus roseus
3.2.1.105 7.015
-
palicoside pH 6.3, 30°C Catharanthus roseus
3.2.1.105 10
-
strictosidine pH 6.3, 30°C Catharanthus roseus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.105 80000
-
2 * 80000, SDS-PAGE Strychnos mellodora
3.2.1.105 80000
-
x * 80000, SDS-PAGE Strychnos mellodora
3.2.1.105 200000
-
native PAGE Strychnos mellodora
3.2.1.105 200000
-
non-denaturing PAGE Strychnos mellodora

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.105 additional information Catharanthus roseus involved In the indole alkaloid biosynthesis by hydrolyzing strictosidine to cathenamine ?
-
?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.105 Catharanthus roseus
-
-
-
3.2.1.105 Strychnos mellodora
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.105
-
Catharanthus roseus
3.2.1.105
-
Strychnos mellodora

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.105 bark
-
Strychnos mellodora
-
3.2.1.105 bark stem Strychnos mellodora
-
3.2.1.105 leaf
-
Strychnos mellodora
-
3.2.1.105 root
-
Strychnos mellodora
-
3.2.1.105 root bark Strychnos mellodora
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.105 dolichantoside + H2O
-
Strychnos mellodora ?
-
?
3.2.1.105 dolichantoside + H2O
-
Catharanthus roseus D-glucose + dolichantoside aglycone
-
?
3.2.1.105 dolichantoside + H2O
-
Strychnos mellodora D-glucose + dolichantoside aglycone
-
?
3.2.1.105 additional information involved In the indole alkaloid biosynthesis by hydrolyzing strictosidine to cathenamine Catharanthus roseus ?
-
?
3.2.1.105 palicoside + H2O
-
Catharanthus roseus ?
-
?
3.2.1.105 palicoside + H2O
-
Strychnos mellodora ?
-
?
3.2.1.105 palicoside + H2O
-
Catharanthus roseus D-glucose + palicoside aglycone
-
?
3.2.1.105 palicoside + H2O
-
Strychnos mellodora D-glucose + palicoside aglycone
-
?
3.2.1.105 strictosidine + H2O
-
Strychnos mellodora D-glucose + strictosidine aglycone
-
?

Subunits

EC Number Subunits Comment Organism
3.2.1.105 ?
-
Catharanthus roseus
3.2.1.105 ? x * 80000, SDS-PAGE Strychnos mellodora
3.2.1.105 dimer 2 * 80000, SDS-PAGE Strychnos mellodora

Synonyms

EC Number Synonyms Comment Organism
3.2.1.105 SGD
-
Catharanthus roseus
3.2.1.105 SGD
-
Strychnos mellodora