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Literature summary extracted from

  • Luijendijk, T.J.C.; Stevens, L.H.; Verpoorte, R.
    Purification and characterization of strictosidine beta-D-glucosidase from Catharanthus roseus cell suspension cultures (1998), Plant Physiol. Biochem., 36, 419-425.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.105 Cu2+ 1 mM Catharanthus roseus
3.2.1.105 serpentine 1 mM Catharanthus roseus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.105 0.018
-
 strictosidine
-
 Catharanthus roseus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.105 63000
-
 x * 63000, SDS-PAGE Catharanthus roseus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.105 strictosidine Catharanthus roseus the enzyme may play an important role in steering the monoterpenoid indole alkaloid biosynthesis in a specific direction ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.105 Catharanthus roseus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.105 3 different enzyme forms Catharanthus roseus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.105 cell suspension culture
-
 Catharanthus roseus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.105 additional information
-
-
 Catharanthus roseus

Storage Stability

EC Number Storage Stability Organism
3.2.1.105 -80°C, 50% loss of activity Catharanthus roseus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.105 strictosidine the enzyme may play an important role in steering the monoterpenoid indole alkaloid biosynthesis in a specific direction Catharanthus roseus ?
-
 ?
3.2.1.105 strictosidine + H2O
-
 Catharanthus roseus D-glucose + strictosidine aglycone
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.105 ? x * 63000, SDS-PAGE Catharanthus roseus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.105 50
-
 stable at Catharanthus roseus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.105 6 8.5
-
 Catharanthus roseus