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Literature summary extracted from

  • Araki, T.; Kitamikado, M.
    Purification and characterization of a novel exo-beta-mannanase from Aeromonas sp. F-25 (1982), J. Biochem., 91, 1181-1186.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.100 Ag+ 1 mM, complete inhibition Aeromonas hydrophila
3.2.1.100 Cu2+ 1 mM, complete inhibition Aeromonas hydrophila
3.2.1.100 EDTA 1 mM, 30-50% inhibition Aeromonas hydrophila
3.2.1.100 Fe3+ 1 mM, 30-50% inhibition Aeromonas hydrophila
3.2.1.100 Hg2+ 1 mM, complete inhibition Aeromonas hydrophila
3.2.1.100 p-chloromercuribenzoate 1 mM, 30-50% inhibition Aeromonas hydrophila
3.2.1.100 Pb2+ 1 mM, 30-50% inhibition Aeromonas hydrophila
3.2.1.100 Zn2+ 1 mM, 30-50% inhibition Aeromonas hydrophila

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.100 0.13
-
 Mannopentaose
-
 Aeromonas hydrophila
3.2.1.100 0.24
-
 Mannotetraose
-
 Aeromonas hydrophila
3.2.1.100 0.51
-
 mannobiose
-
 Aeromonas hydrophila

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.100 extracellular
-
 Aeromonas hydrophila
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.100 64000
-
 gel filtration Aeromonas hydrophila

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.100 Aeromonas hydrophila
-
 ssp. anaerogenes
-
3.2.1.100 Aeromonas hydrophila F-25
-
 ssp. anaerogenes
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.100 F-25 Aeromonas hydrophila

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.100 culture medium
-
 Aeromonas hydrophila
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.100 86.5
-
-
 Aeromonas hydrophila

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.100 codium mannan + H2O
-
 Aeromonas hydrophila mannobiose sole product ?
3.2.1.100 codium mannan + H2O
-
 Aeromonas hydrophila F-25 mannobiose sole product ?
3.2.1.100 coffee mannan + H2O
-
 Aeromonas hydrophila mannobiose sole product ?
3.2.1.100 mannobiosylmannitol + H2O
-
 Aeromonas hydrophila mannobiose + mannitol
-
 ?
3.2.1.100 mannopentaose + H2O
-
 Aeromonas hydrophila mannobiose + mannose
-
 ?
3.2.1.100 mannopentaose + H2O
-
 Aeromonas hydrophila F-25 mannobiose + mannose
-
 ?
3.2.1.100 mannotetraose transglycosylation Aeromonas hydrophila mannohexaose
-
 ?
3.2.1.100 mannotetraose + H2O
-
 Aeromonas hydrophila mannobiose
-
 ?
3.2.1.100 mannotetraose + H2O
-
 Aeromonas hydrophila F-25 mannobiose
-
 ?
3.2.1.100 mannotetraosylmannitol + H2O
-
 Aeromonas hydrophila mannobiose + mannosylmannitol + ?
-
 ?
3.2.1.100 mannotriose + H2O
-
 Aeromonas hydrophila mannobiose + mannose
-
 ?
3.2.1.100 mannotriose + H2O
-
 Aeromonas hydrophila F-25 mannobiose + mannose
-
 ?
3.2.1.100 mannotriose + mannose transglycosylation Aeromonas hydrophila mannopentaose
-
 ?
3.2.1.100 additional information not: mannobiose, p-nitrophenyl-beta-D-mannoside, konjac glucomannan, guar gum galactomannan Aeromonas hydrophila ?
-
 ?
3.2.1.100 additional information not: mannobiose, p-nitrophenyl-beta-D-mannoside, konjac glucomannan, guar gum galactomannan Aeromonas hydrophila F-25 ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.100 37
-
 assay at Aeromonas hydrophila

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.100 45
-
 15 min, stable below Aeromonas hydrophila
3.2.1.100 55
-
 15 min, complete loss of activity Aeromonas hydrophila

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.100 6
-
-
 Aeromonas hydrophila

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.100 5 8.5 20°C, 20 h Aeromonas hydrophila