Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Stevens, R.L.; Fluharty, A.L.; Skokut, M.H.; Kihara, H.
    Purification and properties of arylsulfatase. A from human urine (1975), J. Biol. Chem., 250, 2495-2501.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.6.8 taurodeoxycholate enzyme cleaves cerebroside sulfates only in the presence of either specific detergent, e.g. taurodeoxycholate or human activator protein Homo sapiens

General Stability

EC Number General Stability Organism
3.1.6.8 4°C, addition of dithiothreitol, glutathione, or EDTA reduce storage stability Homo sapiens
3.1.6.8 bovine serum albumin stabilizes Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.6.8 0.2
-
 cerebroside 3-sulfate
-
 Homo sapiens
3.1.6.8 6.7
-
 4-nitrocatechol sulfate
-
 Homo sapiens
3.1.6.8 8
-
 4-Methylumbelliferyl sulfate
-
 Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.6.8 lysosome
-
 Homo sapiens 5764
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.6.8 Mn2+ stimulates Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.6.8 50000
-
 SDS-PAGE Homo sapiens
3.1.6.8 100000
-
 sedimentation equilibrium ultracentrifugation Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.6.8 cerebroside 3-sulfate + H2O Homo sapiens
-
 cerebroside + sulfate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.6.8 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.6.8
-
 Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.6.8 urine
-
 Homo sapiens
-

Storage Stability

EC Number Storage Stability Organism
3.1.6.8 -20°C, protein: 1 mg/ml, stable for months Homo sapiens
3.1.6.8 4°C, pH 7.5, protein: 0.002 mg/ml, bovine serum albumin, insulin or ribonuclease, 15 days, no loss of activity Homo sapiens
3.1.6.8 4°C, pH 7.5, protein: 0.002 mg/ml, bovine serum albumin, insulin or ribonuclease, 8 months, 30% loss of activity Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.6.8 cerebroside 3-sulfate + H2O
-
 Homo sapiens cerebroside + sulfate
-
 ?
3.1.6.8 methylumbelliferyl sulfate + H2O
-
 Homo sapiens methylumbelliferone + sulfate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.6.8 dimer 2 * ca. 55000, SDS-PAGE Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.6.8 0.817
-
 4-Methylumbelliferyl sulfate
-
 Homo sapiens
3.1.6.8 1.45
-
 cerebroside 3-sulfate
-
 Homo sapiens
3.1.6.8 16.8
-
 4-nitrocatechol sulfate
-
 Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.6.8 4.6
-
 cerebroside sulfate Homo sapiens
3.1.6.8 5.2
-
 4-methylumbelliferyl sulfate Homo sapiens
3.1.6.8 5.3
-
 4-nitrocatechol sulfate Homo sapiens