BRENDA - Enzyme Database show

Chemical characterization and substrate specificity of rabbit liver aryl sulfatase A

Waheed, A.; Van Etten, R.L.; Biochim. Biophys. Acta 614, 92-101 (1980)

Data extracted from this reference:

KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.1.6.8
0.06
-
cerebroside sulfate
in presence of 10 mM sodium formate
Oryctolagus cuniculus
3.1.6.8
0.35
-
p-nitrocatechol sulfate
-
Oryctolagus cuniculus
3.1.6.8
3
-
p-nitrophenol sulfate
-
Oryctolagus cuniculus
3.1.6.8
4
-
4-methylumbelliferone sulfate
-
Oryctolagus cuniculus
3.1.6.8
15.9
-
1-naphthyl sulfate
-
Oryctolagus cuniculus
3.1.6.8
31.8
-
Tyrosine O-sulfate
-
Oryctolagus cuniculus
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.1.6.8
lysosome
-
Oryctolagus cuniculus
5764
-
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.1.6.8
140000
-
zonal and frontal gel filtration
Oryctolagus cuniculus
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.1.6.8
cerebroside 3-sulfate + H2O
Oryctolagus cuniculus
-
cerebroside + sulfate
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.1.6.8
Oryctolagus cuniculus
-
-
-
Posttranslational Modification
EC Number
Posttranslational Modification
Commentary
Organism
3.1.6.8
glycoprotein
liver: 4.6% carbohydrate
Oryctolagus cuniculus
Purification (Commentary)
EC Number
Commentary
Organism
3.1.6.8
-
Oryctolagus cuniculus
Reaction
EC Number
Reaction
Commentary
Organism
3.1.6.8
a cerebroside 3-sulfate + H2O = a cerebroside + sulfate
hydrolyses galactose-3-sulfate residues in a number of lipids, also hydrolyses ascorbate 2-sulfate and many phenol sulfates, enzymes catalyze in addition to reaction of EC 3.1.6.8 also arylsulfatase reaction EC 3.1.6.1
Oryctolagus cuniculus
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.6.8
liver
-
Oryctolagus cuniculus
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.6.8
1-naphthyl sulfate + H2O
-
135684
Oryctolagus cuniculus
1-naphthol + sulfate
-
-
-
?
3.1.6.8
4-nitrophenyl sulfate + H2O
-
135684
Oryctolagus cuniculus
4-nitrophenol + sulfate
-
-
-
-
3.1.6.8
ascorbate 2-sulfate + H2O
-
135684
Oryctolagus cuniculus
ascorbate + sulfate
-
-
-
?
3.1.6.8
cerebroside 3-sulfate + H2O
-
135684
Oryctolagus cuniculus
cerebroside + sulfate
-
-
-
?
3.1.6.8
dehydroisoandrosterone sulfate + H2O
-
135684
Oryctolagus cuniculus
dehydroisoandrosterone + sulfate
-
-
-
?
3.1.6.8
estriol 3-sulfate + H2O
-
135684
Oryctolagus cuniculus
estriol + sulfate
-
-
-
?
3.1.6.8
estrone 3-sulfate + H2O
-
135684
Oryctolagus cuniculus
estrone + sulfate
-
-
-
?
3.1.6.8
methylumbelliferyl sulfate + H2O
-
135684
Oryctolagus cuniculus
methylumbelliferone + sulfate
-
-
-
?
3.1.6.8
nitrocatechol sulfate + H2O
-
135684
Oryctolagus cuniculus
nitrocatechol + sulfate
-
-
-
?
3.1.6.8
tyrosine O-sulfate + H2O
-
135684
Oryctolagus cuniculus
tyrosine + sulfate
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
3.1.6.8
monomer
1 * 140000, each monomer consists of 2 equivalent polypeptide chains
Oryctolagus cuniculus
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.1.6.8
37
-
assay
Oryctolagus cuniculus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.1.6.8
0.06
-
cerebroside sulfate
in presence of 10 mM sodium formate
Oryctolagus cuniculus
3.1.6.8
0.35
-
p-nitrocatechol sulfate
-
Oryctolagus cuniculus
3.1.6.8
3
-
p-nitrophenol sulfate
-
Oryctolagus cuniculus
3.1.6.8
4
-
4-methylumbelliferone sulfate
-
Oryctolagus cuniculus
3.1.6.8
15.9
-
1-naphthyl sulfate
-
Oryctolagus cuniculus
3.1.6.8
31.8
-
Tyrosine O-sulfate
-
Oryctolagus cuniculus
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.1.6.8
lysosome
-
Oryctolagus cuniculus
5764
-
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.1.6.8
140000
-
zonal and frontal gel filtration
Oryctolagus cuniculus
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.1.6.8
cerebroside 3-sulfate + H2O
Oryctolagus cuniculus
-
cerebroside + sulfate
-
-
?
Posttranslational Modification (protein specific)
EC Number
Posttranslational Modification
Commentary
Organism
3.1.6.8
glycoprotein
liver: 4.6% carbohydrate
Oryctolagus cuniculus
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.1.6.8
-
Oryctolagus cuniculus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.6.8
liver
-
Oryctolagus cuniculus
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.6.8
1-naphthyl sulfate + H2O
-
135684
Oryctolagus cuniculus
1-naphthol + sulfate
-
-
-
?
3.1.6.8
4-nitrophenyl sulfate + H2O
-
135684
Oryctolagus cuniculus
4-nitrophenol + sulfate
-
-
-
-
3.1.6.8
ascorbate 2-sulfate + H2O
-
135684
Oryctolagus cuniculus
ascorbate + sulfate
-
-
-
?
3.1.6.8
cerebroside 3-sulfate + H2O
-
135684
Oryctolagus cuniculus
cerebroside + sulfate
-
-
-
?
3.1.6.8
dehydroisoandrosterone sulfate + H2O
-
135684
Oryctolagus cuniculus
dehydroisoandrosterone + sulfate
-
-
-
?
3.1.6.8
estriol 3-sulfate + H2O
-
135684
Oryctolagus cuniculus
estriol + sulfate
-
-
-
?
3.1.6.8
estrone 3-sulfate + H2O
-
135684
Oryctolagus cuniculus
estrone + sulfate
-
-
-
?
3.1.6.8
methylumbelliferyl sulfate + H2O
-
135684
Oryctolagus cuniculus
methylumbelliferone + sulfate
-
-
-
?
3.1.6.8
nitrocatechol sulfate + H2O
-
135684
Oryctolagus cuniculus
nitrocatechol + sulfate
-
-
-
?
3.1.6.8
tyrosine O-sulfate + H2O
-
135684
Oryctolagus cuniculus
tyrosine + sulfate
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
3.1.6.8
monomer
1 * 140000, each monomer consists of 2 equivalent polypeptide chains
Oryctolagus cuniculus
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.1.6.8
37
-
assay
Oryctolagus cuniculus