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Literature summary extracted from
- Choline sulfatase of Pseudomonas aeruginosa (1972), Arch. Biochem. Biophys., 153, 664-672.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.6.6 | Choline sulfate | - |
Pseudomonas aeruginosa |  |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.6.6 | betaine | linear competitive inhibitor | Pseudomonas aeruginosa | |
| 3.1.6.6 | choline | partial competitive inhibitor | Penicillium chrysogenum | |
| 3.1.6.6 | choline | uncompetitive inhibitor | Pseudomonas aeruginosa | |
| 3.1.6.6 | Dimethylamine | uncompetitive inhibitor | Pseudomonas aeruginosa | |
| 3.1.6.6 | dimethylaminoethanol | uncompetitive inhibitor | Pseudomonas aeruginosa | |
| 3.1.6.6 | dithiothreitol | - |
Penicillium chrysogenum | |
| 3.1.6.6 | dithiothreitol | after preincubation | Pseudomonas aeruginosa | |
| 3.1.6.6 | mercaptoethanol | - |
Penicillium chrysogenum | |
| 3.1.6.6 | mercaptoethanol | after preincubation | Pseudomonas aeruginosa | |
| 3.1.6.6 | methionine | synthesis of the enzyme is repressed | Pseudomonas aeruginosa | |
| 3.1.6.6 | N-ethylmaleimide | - |
Penicillium chrysogenum | |
| 3.1.6.6 | N-ethylmaleimide | - |
Pseudomonas aeruginosa | |
| 3.1.6.6 | p-chloromercuribenzoate | - |
Penicillium chrysogenum | |
| 3.1.6.6 | p-chloromercuribenzoate | - |
Pseudomonas aeruginosa | |
| 3.1.6.6 | phosphorylcholine | competitive inhibition | Pseudomonas aeruginosa | |
| 3.1.6.6 | tetramethylamine | uncompetitive inhibitor | Pseudomonas aeruginosa | |
| 3.1.6.6 | trimethylamine | uncompetitive inhibitor | Pseudomonas aeruginosa | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.1.6.6 | 1.4 | - |
Choline sulfate | - |
Pseudomonas aeruginosa | |
| 3.1.6.6 | 8.5 | - |
Choline sulfate | - |
Penicillium chrysogenum | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.6.6 | Ca2+ | complete inhibition | Pseudomonas aeruginosa | |
| 3.1.6.6 | Cd2+ | complete inhibition | Pseudomonas aeruginosa | |
| 3.1.6.6 | Co2+ | complete inhibition | Pseudomonas aeruginosa | |
| 3.1.6.6 | Cu2+ | complete inhibition | Pseudomonas aeruginosa | |
| 3.1.6.6 | Mn2+ | complete inhibition | Pseudomonas aeruginosa | |
| 3.1.6.6 | Zn2+ | complete inhibition | Pseudomonas aeruginosa | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.1.6.6 | 175000 | - |
gel filtration | Pseudomonas aeruginosa |
| 3.1.6.6 | 345000 | - |
gel filtration | Penicillium chrysogenum |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.6.6 | choline sulfate + H2O | Penicillium chrysogenum | - |
choline + sulfate | - |
? | |
| 3.1.6.6 | choline sulfate + H2O | Pseudomonas aeruginosa | substrate-induced | choline + sulfate | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.1.6.6 | Penicillium chrysogenum | - |
- |
- |
| 3.1.6.6 | Pseudomonas aeruginosa | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.1.6.6 | partial | Pseudomonas aeruginosa |
Reaction
| EC Number | Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|---|
| 3.1.6.6 | choline sulfate + H2O = choline + sulfate | ordered bi-bi mechanism | Penicillium chrysogenum | |
| 3.1.6.6 | choline sulfate + H2O = choline + sulfate | ordered bi-bi mechanism | Pseudomonas aeruginosa |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.1.6.6 | 0.262 | - |
- |
Pseudomonas aeruginosa |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.6.6 | choline sulfate + H2O | - |
Penicillium chrysogenum | choline + sulfate | - |
? | |
| 3.1.6.6 | choline sulfate + H2O | reverse reaction not demonstrated | Penicillium chrysogenum | choline + sulfate | - |
ir | |
| 3.1.6.6 | choline sulfate + H2O | reverse reaction not demonstrated | Pseudomonas aeruginosa | choline + sulfate | - |
ir | |
| 3.1.6.6 | choline sulfate + H2O | substrate-induced | Pseudomonas aeruginosa | choline + sulfate | - |
? | |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.1.6.6 | 30 | - |
- |
Pseudomonas aeruginosa |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.6.6 | 7.5 | - |
- |
Pseudomonas aeruginosa |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.6.6 | 6 | 10 | - |
Pseudomonas aeruginosa |
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