BRENDA - Enzyme Database show

Isolation and characterization of a new enzyme choline sulfatase

Takebe, I.; J. Biochem. 50, 245-255 (1961)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
3.1.6.6
Choline sulfate
-
Pseudomonas nitroreducens
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.6.6
choline chloride
some inhibition at higher concentration
Pseudomonas nitroreducens
3.1.6.6
F-
partial inhibition
Pseudomonas nitroreducens
3.1.6.6
sulfite
-
Pseudomonas nitroreducens
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.1.6.6
40
-
Choline sulfate
-
Pseudomonas nitroreducens
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
3.1.6.6
Ca2+
slight inhibition
Pseudomonas nitroreducens
3.1.6.6
Co2+
complete inhibition
Pseudomonas nitroreducens
3.1.6.6
Cu2+
complete inhibition
Pseudomonas nitroreducens
3.1.6.6
Hg2+
complete inhibition
Pseudomonas nitroreducens
3.1.6.6
Mg2+
slight inhibition
Pseudomonas nitroreducens
3.1.6.6
Mn2+
slight inhibition
Pseudomonas nitroreducens
3.1.6.6
Zn2+
complete inhibition
Pseudomonas nitroreducens
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.1.6.6
choline sulfate + H2O
Pseudomonas nitroreducens
substrate-induced
choline + sulfate
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.1.6.6
Pseudomonas nitroreducens
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
3.1.6.6
partial
Pseudomonas nitroreducens
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.1.6.6
0.0714
-
-
Pseudomonas nitroreducens
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.6.6
amylose polysulfate + H2O
slightly hydrolyzed
135658
Pseudomonas nitroreducens
amylose + sulfate
-
-
-
?
3.1.6.6
choline sulfate + H2O
-
135658
Pseudomonas nitroreducens
choline + sulfate
-
-
-
-
3.1.6.6
choline sulfate + H2O
substrate-induced
135658
Pseudomonas nitroreducens
choline + sulfate
-
-
-
?
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.1.6.6
37
-
assay
Pseudomonas nitroreducens
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.6.6
8.3
-
-
Pseudomonas nitroreducens
pH Stability
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
3.1.6.6
6
10
inactvation af the enzyme at lower pH's, pH 10: about 50% of activity maximum; most stable at pH 6.6
Pseudomonas nitroreducens
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
3.1.6.6
Choline sulfate
-
Pseudomonas nitroreducens
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.6.6
choline chloride
some inhibition at higher concentration
Pseudomonas nitroreducens
3.1.6.6
F-
partial inhibition
Pseudomonas nitroreducens
3.1.6.6
sulfite
-
Pseudomonas nitroreducens
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.1.6.6
40
-
Choline sulfate
-
Pseudomonas nitroreducens
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
3.1.6.6
Ca2+
slight inhibition
Pseudomonas nitroreducens
3.1.6.6
Co2+
complete inhibition
Pseudomonas nitroreducens
3.1.6.6
Cu2+
complete inhibition
Pseudomonas nitroreducens
3.1.6.6
Hg2+
complete inhibition
Pseudomonas nitroreducens
3.1.6.6
Mg2+
slight inhibition
Pseudomonas nitroreducens
3.1.6.6
Mn2+
slight inhibition
Pseudomonas nitroreducens
3.1.6.6
Zn2+
complete inhibition
Pseudomonas nitroreducens
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.1.6.6
choline sulfate + H2O
Pseudomonas nitroreducens
substrate-induced
choline + sulfate
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.1.6.6
partial
Pseudomonas nitroreducens
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.1.6.6
0.0714
-
-
Pseudomonas nitroreducens
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.6.6
amylose polysulfate + H2O
slightly hydrolyzed
135658
Pseudomonas nitroreducens
amylose + sulfate
-
-
-
?
3.1.6.6
choline sulfate + H2O
-
135658
Pseudomonas nitroreducens
choline + sulfate
-
-
-
-
3.1.6.6
choline sulfate + H2O
substrate-induced
135658
Pseudomonas nitroreducens
choline + sulfate
-
-
-
?
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.1.6.6
37
-
assay
Pseudomonas nitroreducens
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.6.6
8.3
-
-
Pseudomonas nitroreducens
pH Stability (protein specific)
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
3.1.6.6
6
10
inactvation af the enzyme at lower pH's, pH 10: about 50% of activity maximum; most stable at pH 6.6
Pseudomonas nitroreducens