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Literature summary extracted from

  • Roberton, A.M.; McKenzie, C.G.; Sharff, N.; Stubbs, L.B.
    A glycosulphatase that removes sulphate from mucus glycoprotein (1993), Biochem. J., 293, 683-689.
No PubMed abstract available

General Stability

EC Number General Stability Organism
3.1.6.3 unstable to freezing Prevotella sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.6.3 HgCl2 weak Prevotella sp.
3.1.6.3 additional information no inhibition by iodacetamide Prevotella sp.
3.1.6.3 p-hydroxymercuribenzoate
-
 Prevotella sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.6.3 60
-
 glucose 6-sulfate
-
 Prevotella sp.

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.6.3 periplasm
-
 Prevotella sp.
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.6.3 58000
-
 2 * 58000, SDS-PAGE Prevotella sp.
3.1.6.3 111000
-
 gel filtration Prevotella sp.

Organism

EC Number Organism UniProt Comment Textmining
3.1.6.3 Prevotella sp.
-
-
-
3.1.6.3 Prevotella sp. RS2
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.6.3
-
 Prevotella sp.

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.6.3 colon
-
 Prevotella sp.
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.6.3 1.9
-
-
 Prevotella sp.

Storage Stability

EC Number Storage Stability Organism
3.1.6.3 4°C, 20 mM phosphate buffer, pH 7.4, 2-mercaptoethanol, 1-3 months, 92-74% of original activity Prevotella sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.6.3 D-glucose-6-sulfate + H2O
-
 Prevotella sp. D-glucose + sulfate
-
 ?
3.1.6.3 D-glucose-6-sulfate + H2O
-
 Prevotella sp. RS2 D-glucose + sulfate
-
 ?
3.1.6.3 N-acetyl-D-glucosamine 6-sulfate + H2O
-
 Prevotella sp. N-acetyl-D-glucosamine + sulfate
-
 ?
3.1.6.3 N-acetyl-D-glucosamine 6-sulfate + H2O
-
 Prevotella sp. RS2 N-acetyl-D-glucosamine + sulfate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.6.3 dimer 2 * 58000, SDS-PAGE Prevotella sp.