BRENDA - Enzyme Database show

Steroid sulphatase: expression, isolation and inhibition for active-site identification studies

Purohit, A.; Potter, B.V.; Parker, M.G.; Reed, M.J.; Chem. Biol. Interact. 109, 183-193 (1998)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
3.1.6.2
expression in Escherichia coli and CHO cells
Homo sapiens
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.6.2
estrone-3-O-sulfamate
active site-directed inhibitor; EMATE, most potent, irreversible
Helix pomatia
3.1.6.2
estrone-3-O-sulfamate
active site-directed inhibitor; EMATE, most potent, irreversible
Homo sapiens
3.1.6.2
Rose bengal
histidine modification
Helix pomatia
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.1.6.2
steroid sulfates + H2O
Homo sapiens
also acts on some related steryl sulfates, phenol sulfates, steroid arylsulfates and steroid alkyl sulfates catalyzed at the same site
steroid + sulfate
-
-
?
3.1.6.2
steroid sulfates + H2O
Helix pomatia
also acts on some related steryl sulfates, phenol sulfates, steroid arylsulfates and steroid alkyl sulfates catalyzed at the same site
steroid + sulfate
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.1.6.2
Helix pomatia
-
-
-
3.1.6.2
Homo sapiens
-
-
-
Posttranslational Modification
EC Number
Posttranslational Modification
Commentary
Organism
3.1.6.2
glycoprotein
is glycosylated, binds to concanavalin A
Helix pomatia
3.1.6.2
glycoprotein
-
Homo sapiens
Purification (Commentary)
EC Number
Commentary
Organism
3.1.6.2
of the recombinant protein
Homo sapiens
3.1.6.2
partial, the soluble enzyme
Helix pomatia
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.6.2
placenta
-
Homo sapiens
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.6.2
dehydroepiandrosterone 3-sulfate + H2O
-
135624
Homo sapiens
dehydroepiandrosterone + sulfate
-
-
-
?
3.1.6.2
dehydroepiandrosterone 3-sulfate + H2O
-
135624
Helix pomatia
dehydroepiandrosterone + sulfate
-
-
-
?
3.1.6.2
estrone sulfate + H2O
-
135624
Homo sapiens
estrone + sulfate
-
-
-
?
3.1.6.2
estrone sulfate + H2O
-
135624
Helix pomatia
estrone + sulfate
-
-
-
-
3.1.6.2
steroid sulfates + H2O
also acts on some related steryl sulfates, phenol sulfates, steroid arylsulfates and steroid alkyl sulfates catalyzed at the same site
135624
Homo sapiens
steroid + sulfate
-
-
-
?
3.1.6.2
steroid sulfates + H2O
also acts on some related steryl sulfates, phenol sulfates, steroid arylsulfates and steroid alkyl sulfates catalyzed at the same site
135624
Helix pomatia
steroid + sulfate
-
-
-
?
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
3.1.6.2
expression in Escherichia coli and CHO cells
Homo sapiens
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.6.2
estrone-3-O-sulfamate
active site-directed inhibitor; EMATE, most potent, irreversible
Helix pomatia
3.1.6.2
estrone-3-O-sulfamate
active site-directed inhibitor; EMATE, most potent, irreversible
Homo sapiens
3.1.6.2
Rose bengal
histidine modification
Helix pomatia
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.1.6.2
steroid sulfates + H2O
Homo sapiens
also acts on some related steryl sulfates, phenol sulfates, steroid arylsulfates and steroid alkyl sulfates catalyzed at the same site
steroid + sulfate
-
-
?
3.1.6.2
steroid sulfates + H2O
Helix pomatia
also acts on some related steryl sulfates, phenol sulfates, steroid arylsulfates and steroid alkyl sulfates catalyzed at the same site
steroid + sulfate
-
-
?
Posttranslational Modification (protein specific)
EC Number
Posttranslational Modification
Commentary
Organism
3.1.6.2
glycoprotein
is glycosylated, binds to concanavalin A
Helix pomatia
3.1.6.2
glycoprotein
-
Homo sapiens
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.1.6.2
of the recombinant protein
Homo sapiens
3.1.6.2
partial, the soluble enzyme
Helix pomatia
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.6.2
placenta
-
Homo sapiens
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.6.2
dehydroepiandrosterone 3-sulfate + H2O
-
135624
Homo sapiens
dehydroepiandrosterone + sulfate
-
-
-
?
3.1.6.2
dehydroepiandrosterone 3-sulfate + H2O
-
135624
Helix pomatia
dehydroepiandrosterone + sulfate
-
-
-
?
3.1.6.2
estrone sulfate + H2O
-
135624
Homo sapiens
estrone + sulfate
-
-
-
?
3.1.6.2
estrone sulfate + H2O
-
135624
Helix pomatia
estrone + sulfate
-
-
-
-
3.1.6.2
steroid sulfates + H2O
also acts on some related steryl sulfates, phenol sulfates, steroid arylsulfates and steroid alkyl sulfates catalyzed at the same site
135624
Homo sapiens
steroid + sulfate
-
-
-
?
3.1.6.2
steroid sulfates + H2O
also acts on some related steryl sulfates, phenol sulfates, steroid arylsulfates and steroid alkyl sulfates catalyzed at the same site
135624
Helix pomatia
steroid + sulfate
-
-
-
?