BRENDA - Enzyme Database show

Purification and properties of arylsulfatase C from human placenta

Vaccaro, A.M.; Salvioli, R.; Muscillo, M.; Renola, L.; Enzyme 37, 115-126 (1987)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.6.2
dehydroepiandrosterone
-
Homo sapiens
3.1.6.2
Estrone 3-sulfate
competitive inhibitor
Homo sapiens
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.1.6.2
0.05
-
dehydroepiandrosterone 3-sulfate
-
Homo sapiens
3.1.6.2
0.1
-
methylumbelliferyl sulfate
esterone 3-sulfate
Homo sapiens
3.1.6.2
1
-
p-nitrophenylsulfate
-
Homo sapiens
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.1.6.2
78000
-
1 * 78000, solubilized in Triton X-100; alpha, 3 * 78000, SDS-PAGE,solubilized in Triton X-100
Homo sapiens
3.1.6.2
238000
-
gel filtration
Homo sapiens
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.1.6.2
steroid sulfates + H2O
Homo sapiens
also acts on some related steryl sulfates, phenol sulfates, steroid arylsulfates and steroid alkyl sulfates catalyzed at the same site
steroid + sulfate
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.1.6.2
Homo sapiens
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
3.1.6.2
-
Homo sapiens
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.6.2
placenta
-
Homo sapiens
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.6.2
dehydroepiandrosterone 3-sulfate + H2O
-
135604
Homo sapiens
dehydroepiandrosterone + sulfate
-
-
-
?
3.1.6.2
estrone sulfate + H2O
-
135604
Homo sapiens
estrone + sulfate
-
-
-
?
3.1.6.2
p-nitrophenyl-sulfate + H2O
arylsulfatase activity, EC 3.1.6.1, and various steroid sulfatase activities (EC 3.1.6.1) are functions of the same protein
135604
Homo sapiens
p-nitrophenol + sulfate
-
-
-
?
3.1.6.2
steroid sulfates + H2O
also acts on some related steryl sulfates, phenol sulfates, steroid arylsulfates and steroid alkyl sulfates catalyzed at the same site
135604
Homo sapiens
steroid + sulfate
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
3.1.6.2
monomer
1 * 78000, solubilized in Triton X-100
Homo sapiens
3.1.6.2
trimer
alpha, 3 * 78000, SDS-PAGE,solubilized in Triton X-100
Homo sapiens
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
3.1.6.2
37
-
5 h, 20% loss of activity
Homo sapiens
3.1.6.2
50
-
3 h, 50% loss of activity
Homo sapiens
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.6.2
6.8
-
4-methylumbelliferyl sulfate
Homo sapiens
3.1.6.2
7.3
-
p-nitrophenyl sulfate
Homo sapiens
3.1.6.2
8
-
dehydroepiandrosterone 3-sulfate
Homo sapiens
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.6.2
dehydroepiandrosterone
-
Homo sapiens
3.1.6.2
Estrone 3-sulfate
competitive inhibitor
Homo sapiens
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.1.6.2
0.05
-
dehydroepiandrosterone 3-sulfate
-
Homo sapiens
3.1.6.2
0.1
-
methylumbelliferyl sulfate
esterone 3-sulfate
Homo sapiens
3.1.6.2
1
-
p-nitrophenylsulfate
-
Homo sapiens
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.1.6.2
78000
-
1 * 78000, solubilized in Triton X-100; alpha, 3 * 78000, SDS-PAGE,solubilized in Triton X-100
Homo sapiens
3.1.6.2
238000
-
gel filtration
Homo sapiens
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.1.6.2
steroid sulfates + H2O
Homo sapiens
also acts on some related steryl sulfates, phenol sulfates, steroid arylsulfates and steroid alkyl sulfates catalyzed at the same site
steroid + sulfate
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.1.6.2
-
Homo sapiens
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.6.2
placenta
-
Homo sapiens
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.6.2
dehydroepiandrosterone 3-sulfate + H2O
-
135604
Homo sapiens
dehydroepiandrosterone + sulfate
-
-
-
?
3.1.6.2
estrone sulfate + H2O
-
135604
Homo sapiens
estrone + sulfate
-
-
-
?
3.1.6.2
p-nitrophenyl-sulfate + H2O
arylsulfatase activity, EC 3.1.6.1, and various steroid sulfatase activities (EC 3.1.6.1) are functions of the same protein
135604
Homo sapiens
p-nitrophenol + sulfate
-
-
-
?
3.1.6.2
steroid sulfates + H2O
also acts on some related steryl sulfates, phenol sulfates, steroid arylsulfates and steroid alkyl sulfates catalyzed at the same site
135604
Homo sapiens
steroid + sulfate
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
3.1.6.2
monomer
1 * 78000, solubilized in Triton X-100
Homo sapiens
3.1.6.2
trimer
alpha, 3 * 78000, SDS-PAGE,solubilized in Triton X-100
Homo sapiens
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
3.1.6.2
37
-
5 h, 20% loss of activity
Homo sapiens
3.1.6.2
50
-
3 h, 50% loss of activity
Homo sapiens
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.6.2
6.8
-
4-methylumbelliferyl sulfate
Homo sapiens
3.1.6.2
7.3
-
p-nitrophenyl sulfate
Homo sapiens
3.1.6.2
8
-
dehydroepiandrosterone 3-sulfate
Homo sapiens