BRENDA - Enzyme Database show

Biochemical characterization of arylsulfatase-C isozymes in human fibroblasts

Simard, J.P.S.; Ameen, M.; Chang, P.L.; Biochem. Biophys. Res. Commun. 128, 1388-1394 (1985)

Data extracted from this reference:

Application
EC Number
Application
Commentary
Organism
3.1.6.2
medicine
-
Homo sapiens
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.1.6.2
0.005
-
esterone 3-sulfate
-
Homo sapiens
3.1.6.2
0.217
-
4-methylumbelliferylsulfate
-
Homo sapiens
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.1.6.2
steroid sulfates + H2O
Homo sapiens
also acts on some related steryl sulfates, phenol sulfates, steroid arylsulfates and steroid alkyl sulfates catalyzed at the same site
steroid + sulfate
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.1.6.2
Homo sapiens
-
2 forms of arylsulfatase C: slow form, fast form, only slow form is associated with sterol sulfatase activity; fast form in patients with X-linked ichthyosis
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.6.2
skin
cultured fibroblasts
Homo sapiens
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.6.2
4-methylumbelliferyl sulfate + H2O
-
135600
Homo sapiens
4-methylumbelliferone + sulfate
-
-
-
?
3.1.6.2
dehydroisoandrosterone 3-sulfate + H2O
-
135600
Homo sapiens
dehydroisoandrosterone + sulfate
-
-
-
?
3.1.6.2
estrone sulfate + H2O
-
135600
Homo sapiens
estrone + sulfate
-
-
-
?
3.1.6.2
steroid sulfates + H2O
also acts on some related steryl sulfates, phenol sulfates, steroid arylsulfates and steroid alkyl sulfates catalyzed at the same site
135600
Homo sapiens
steroid + sulfate
-
-
-
?
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
3.1.6.2
60
-
10 min, 50% loss of activity
Homo sapiens
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.6.2
7.7
-
fast form
Homo sapiens
3.1.6.2
8
-
slow form
Homo sapiens
Application (protein specific)
EC Number
Application
Commentary
Organism
3.1.6.2
medicine
-
Homo sapiens
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.1.6.2
0.005
-
esterone 3-sulfate
-
Homo sapiens
3.1.6.2
0.217
-
4-methylumbelliferylsulfate
-
Homo sapiens
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.1.6.2
steroid sulfates + H2O
Homo sapiens
also acts on some related steryl sulfates, phenol sulfates, steroid arylsulfates and steroid alkyl sulfates catalyzed at the same site
steroid + sulfate
-
-
?
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.6.2
skin
cultured fibroblasts
Homo sapiens
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.6.2
4-methylumbelliferyl sulfate + H2O
-
135600
Homo sapiens
4-methylumbelliferone + sulfate
-
-
-
?
3.1.6.2
dehydroisoandrosterone 3-sulfate + H2O
-
135600
Homo sapiens
dehydroisoandrosterone + sulfate
-
-
-
?
3.1.6.2
estrone sulfate + H2O
-
135600
Homo sapiens
estrone + sulfate
-
-
-
?
3.1.6.2
steroid sulfates + H2O
also acts on some related steryl sulfates, phenol sulfates, steroid arylsulfates and steroid alkyl sulfates catalyzed at the same site
135600
Homo sapiens
steroid + sulfate
-
-
-
?
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
3.1.6.2
60
-
10 min, 50% loss of activity
Homo sapiens
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.6.2
7.7
-
fast form
Homo sapiens
3.1.6.2
8
-
slow form
Homo sapiens