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Literature summary extracted from
- Purification and characterization of N-acetylglucosamine-6-sulfate sulfatase from bovine kidney: evidence for the presence of a novel endosulfatase activity (1994), Biochemistry, 33, 4273-4282.
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 3.1.6.14 | unstable to freezing, thawing | Bos taurus |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.6.14 | PO43- | - |
Bos taurus |  |
| 3.1.6.14 | SO42- | - |
Bos taurus | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.1.6.14 | 4.2 | - |
N-acetyl-D-glucosamine 6-sulfate | - |
Bos taurus | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.1.6.14 | 52500 | - |
SDS-PAGE, reduced | Bos taurus |
| 3.1.6.14 | 57800 | - |
SDS-PAGE, nonreduced | Bos taurus |
| 3.1.6.14 | 60200 | - |
gel filtration | Bos taurus |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.6.14 | N-acetyl-D-glucosamine 6-sulfate units + H2O | Bos taurus | - |
N-acetyl-D-glucosamine units + sulfate | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.1.6.14 | Bos taurus | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.1.6.14 | to homogeneity | Bos taurus |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.1.6.14 | kidney | - |
Bos taurus | - |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 3.1.6.14 | -80°C, pH 5.5, 0.02% NaN3, several months, no loss of activity | Bos taurus |
| 3.1.6.14 | 4°C, pH 5.5, 0.02% NaN3, 1 month | Bos taurus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.6.14 | N-acetyl-D-glucosamine 6-sulfate units + H2O | - |
Bos taurus | N-acetyl-D-glucosamine units + sulfate | - |
? | |
| 3.1.6.14 | N-acetyl-D-glucosamine 6-sulfate units + H2O | acts also as endosulfatase | Bos taurus | N-acetyl-D-glucosamine units + sulfate | - |
? | |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.1.6.14 | 37 | - |
- |
Bos taurus |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.1.6.14 | 15 | - |
no activity | Bos taurus |
| 3.1.6.14 | 60 | - |
no activity | Bos taurus |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.6.14 | 6.5 | - |
- |
Bos taurus |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.6.14 | 4 | 9 | - |
Bos taurus |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.6.14 | 4 | 9 | no activity below pH 4 or above pH 9 | Bos taurus |
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Release 2023.1 (January 2023)
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