Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Tsuji, M.; Nakanishi, Y.; Habuchi, H.; Ishihara, K.; Suzuki, S.
    The common identity of UDP-N-acetylgalactosamine 4-sulfatase, nitrocatechol sulfatase (arylsulfatase), and chondroitin 4-sulfatase (1980), Biochim. Biophys. Acta, 612, 373-383.
    View publication on PubMed

General Stability

EC Number General Stability Organism
3.1.6.12 thawing and freezing, stable at -20°C, 6 months with occasional thawing and refreezing Gallus gallus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.6.12 Na2HPO4
-
 Gallus gallus
3.1.6.12 SO42-
-
 Gallus gallus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.6.12 0.43
-
 UDP-N-acetylgalactosamine 4-sulfate
-
 Gallus gallus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.6.12 47000
-
 gel filtration Gallus gallus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.6.12 N-acetyl-D-galactosamine 4-sulfate units + H2O Gallus gallus acts also on N-acetylglucosamine 4-sulfate N-acetyl-D-galactosamine units + sulfate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.6.12 Gallus gallus
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.6.12 oviduct
-
 Gallus gallus
-

Storage Stability

EC Number Storage Stability Organism
3.1.6.12 -20°C, 6 months, with occasional thawing and refreezing, no loss of activity Gallus gallus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.6.12 chondroitin 4-sulfate + H2O
-
 Gallus gallus chondroitin + sulfate
-
 ?
3.1.6.12 dermatan sulfate + H2O
-
 Gallus gallus dermatan + sulfate
-
 ?
3.1.6.12 N-acetyl-D-galactosamine 4-sulfate + H2O
-
 Gallus gallus N-acetyl-D-galactosamine + sulfate
-
 ?
3.1.6.12 N-acetyl-D-galactosamine 4-sulfate units + H2O acts also on N-acetylglucosamine 4-sulfate Gallus gallus N-acetyl-D-galactosamine units + sulfate
-
 ?
3.1.6.12 N-acetylgalactosamine 4,6-bisulfate-(beta-glucuronosyl-(1-3)-N-acetylgalactosamine 4-sulfate)3 + H2O hydrolysis only at the nonreducing residues Gallus gallus N-acetylgalactosamine 6-sulfate-(beta-glucuronosyl-(1-3)-N-acetylgalactosamine)3 + sulfate
-
 ?
3.1.6.12 p-nitrocatechol sulfate + H2O enzymes catalyze in addition to reaction of EC 3.1.6.12 also arylsulfatase reaction , see also EC 3.1.6.1 Gallus gallus p-nitrocatechol + sulfate
-
 ?
3.1.6.12 p-nitrophenyl sulfate + H2O
-
 Gallus gallus p-nitrophenol + sulfate
-
 ?
3.1.6.12 UDP-N-acetylgalactosamine 4,6-bisulfate + H2O
-
 Gallus gallus UDP-N-acetylgalactosamine 6-sulfate + sulfate
-
 ?
3.1.6.12 UDP-N-acetylgalactosamine-4-sulfate + H2O
-
 Gallus gallus UDP-N-acetylgalactosamine + sulfate
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.6.12 37
-
 assay at Gallus gallus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.6.12 60
-
 20 min Gallus gallus
3.1.6.12 60
-
 75% loss of activity Gallus gallus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.6.12 4.8
-
 chondroitin 4-sulfate Gallus gallus
3.1.6.12 5
-
 UDP-N-acetylgalactosamine 4-sulfate Gallus gallus
3.1.6.12 5.6
-
 nitrocatechol sulfate Gallus gallus