BRENDA - Enzyme Database show

Arylsulfatase from Streptomyces griseorubiginosus S980-14

Ueki, T.; Sawada, Y.; Fukagawa, Y.; Oki, T.; Biosci. Biotechnol. Biochem. 59, 1062-1068 (1995)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.6.1
(NH4)2SO4
-
Streptomyces griseorubiginosus
3.1.6.1
1,3-phenanthroline
-
Streptomyces griseorubiginosus
3.1.6.1
AlCl3
-
Streptomyces griseorubiginosus
3.1.6.1
BaCl2
-
Streptomyces griseorubiginosus
3.1.6.1
citrate
-
Streptomyces griseorubiginosus
3.1.6.1
CoCl2
-
Streptomyces griseorubiginosus
3.1.6.1
CuCl2
-
Streptomyces griseorubiginosus
3.1.6.1
Cys
-
Streptomyces griseorubiginosus
3.1.6.1
dithiothreitol
-
Streptomyces griseorubiginosus
3.1.6.1
EDTA
-
Streptomyces griseorubiginosus
3.1.6.1
FeCl2
-
Streptomyces griseorubiginosus
3.1.6.1
FeCl3
-
Streptomyces griseorubiginosus
3.1.6.1
Hg2+
HgCl2
Streptomyces griseorubiginosus
3.1.6.1
iodoacetate
-
Streptomyces griseorubiginosus
3.1.6.1
K2HPO4
-
Streptomyces griseorubiginosus
3.1.6.1
K2SO3
-
Streptomyces griseorubiginosus
3.1.6.1
KCl
-
Streptomyces griseorubiginosus
3.1.6.1
MnCl2
-
Streptomyces griseorubiginosus
3.1.6.1
NaCl
-
Streptomyces griseorubiginosus
3.1.6.1
NEM
-
Streptomyces griseorubiginosus
3.1.6.1
NiCl2
-
Streptomyces griseorubiginosus
3.1.6.1
p-(amidinophenyl)methanesulfonyl fluoride
-
Streptomyces griseorubiginosus
3.1.6.1
PCMB
-
Streptomyces griseorubiginosus
3.1.6.1
SO42-
K2SO4
Streptomyces griseorubiginosus
3.1.6.1
Zn2+
ZnCl2
Streptomyces griseorubiginosus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.1.6.1
0.0037
-
4-nitrophenyl sulfate
in presence of 1 mM CaCl2
Streptomyces griseorubiginosus
3.1.6.1
0.017
-
4-nitrophenyl sulfate
in absence CaCl2
Streptomyces griseorubiginosus
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
3.1.6.1
Ca2+
required
Streptomyces griseorubiginosus
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.1.6.1
Streptomyces griseorubiginosus
-
arylsulfatase Es-1
-
3.1.6.1
Streptomyces griseorubiginosus S980-14
-
arylsulfatase Es-1
-
Purification (Commentary)
EC Number
Commentary
Organism
3.1.6.1
arylsulfatase Es-1
Streptomyces griseorubiginosus
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.1.6.1
1.14
-
-
Streptomyces griseorubiginosus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.6.1
2'',3''-di-O-acetyletoposide 4'-sulfate + H2O
-
135518
Streptomyces griseorubiginosus
2'',3''-di-O-acetyletoposide + sulfate
-
-
-
?
3.1.6.1
4-acetylphenyl sulfate + H2O
-
135518
Streptomyces griseorubiginosus
4-acetylphenol + sulfate
-
-
-
?
3.1.6.1
4-acetylphenyl sulfate + H2O
-
135518
Streptomyces griseorubiginosus S980-14
4-acetylphenol + sulfate
-
-
-
?
3.1.6.1
4-methylumbelliferyl sulfate + H2O
-
135518
Streptomyces griseorubiginosus
4-methylumbelliferone + sulfate
-
-
-
?
3.1.6.1
4-methylumbelliferyl sulfate + H2O
-
135518
Streptomyces griseorubiginosus S980-14
4-methylumbelliferone + sulfate
-
-
-
?
3.1.6.1
4-nitrocatechol sulfate + H2O
-
135518
Streptomyces griseorubiginosus
4-nitrocatechol + sulfate
-
-
-
?
3.1.6.1
4-nitrocatechol sulfate + H2O
-
135518
Streptomyces griseorubiginosus S980-14
4-nitrocatechol + sulfate
-
-
-
?
3.1.6.1
4-nitrophenyl sulfate + H2O
-
135518
Streptomyces griseorubiginosus
4-nitrophenol + sulfate
-
-
-
?
3.1.6.1
4-nitrophenyl sulfate + H2O
-
135518
Streptomyces griseorubiginosus S980-14
4-nitrophenol + sulfate
-
-
-
?
3.1.6.1
androsterone sulfate + H2O
-
135518
Streptomyces griseorubiginosus
androsterone + sulfate
-
-
-
?
3.1.6.1
chondroitin sulfate C + H2O
-
135518
Streptomyces griseorubiginosus
chondroitin + sulfate
-
-
-
?
3.1.6.1
estrone 3-sulfate + H2O
-
135518
Streptomyces griseorubiginosus
sulfate + estrone
-
-
-
?
3.1.6.1
estrone 3-sulfate + H2O
-
135518
Streptomyces griseorubiginosus S980-14
sulfate + estrone
-
-
-
?
3.1.6.1
etoposide 4'-sulfate + H2O
-
135518
Streptomyces griseorubiginosus
etoposide + sulfate
-
-
-
?
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.1.6.1
37
-
-
Streptomyces griseorubiginosus
Temperature Range [°C]
EC Number
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
3.1.6.1
4
50
4°C: about 40% of maximal activity, 50°C: about 45% of maximal activity
Streptomyces griseorubiginosus
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
3.1.6.1
40
-
30 min, stable below
Streptomyces griseorubiginosus
3.1.6.1
50
-
30 min, about 50% loss of activity
Streptomyces griseorubiginosus
3.1.6.1
70
-
30 min, complete loss of activity
Streptomyces griseorubiginosus
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.6.1
8.5
-
-
Streptomyces griseorubiginosus
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
3.1.6.1
7.4
10
pH 7.4: about 35% of maximal activity, 10.0: about 50% of maximal activity
Streptomyces griseorubiginosus
pH Stability
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
3.1.6.1
8
9.5
37°C, 30 min, most stable
Streptomyces griseorubiginosus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.6.1
(NH4)2SO4
-
Streptomyces griseorubiginosus
3.1.6.1
1,3-phenanthroline
-
Streptomyces griseorubiginosus
3.1.6.1
AlCl3
-
Streptomyces griseorubiginosus
3.1.6.1
BaCl2
-
Streptomyces griseorubiginosus
3.1.6.1
citrate
-
Streptomyces griseorubiginosus
3.1.6.1
CoCl2
-
Streptomyces griseorubiginosus
3.1.6.1
CuCl2
-
Streptomyces griseorubiginosus
3.1.6.1
Cys
-
Streptomyces griseorubiginosus
3.1.6.1
dithiothreitol
-
Streptomyces griseorubiginosus
3.1.6.1
EDTA
-
Streptomyces griseorubiginosus
3.1.6.1
FeCl2
-
Streptomyces griseorubiginosus
3.1.6.1
FeCl3
-
Streptomyces griseorubiginosus
3.1.6.1
Hg2+
HgCl2
Streptomyces griseorubiginosus
3.1.6.1
iodoacetate
-
Streptomyces griseorubiginosus
3.1.6.1
K2HPO4
-
Streptomyces griseorubiginosus
3.1.6.1
K2SO3
-
Streptomyces griseorubiginosus
3.1.6.1
KCl
-
Streptomyces griseorubiginosus
3.1.6.1
MnCl2
-
Streptomyces griseorubiginosus
3.1.6.1
NaCl
-
Streptomyces griseorubiginosus
3.1.6.1
NEM
-
Streptomyces griseorubiginosus
3.1.6.1
NiCl2
-
Streptomyces griseorubiginosus
3.1.6.1
p-(amidinophenyl)methanesulfonyl fluoride
-
Streptomyces griseorubiginosus
3.1.6.1
PCMB
-
Streptomyces griseorubiginosus
3.1.6.1
SO42-
K2SO4
Streptomyces griseorubiginosus
3.1.6.1
Zn2+
ZnCl2
Streptomyces griseorubiginosus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.1.6.1
0.0037
-
4-nitrophenyl sulfate
in presence of 1 mM CaCl2
Streptomyces griseorubiginosus
3.1.6.1
0.017
-
4-nitrophenyl sulfate
in absence CaCl2
Streptomyces griseorubiginosus
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
3.1.6.1
Ca2+
required
Streptomyces griseorubiginosus
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.1.6.1
arylsulfatase Es-1
Streptomyces griseorubiginosus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.1.6.1
1.14
-
-
Streptomyces griseorubiginosus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.6.1
2'',3''-di-O-acetyletoposide 4'-sulfate + H2O
-
135518
Streptomyces griseorubiginosus
2'',3''-di-O-acetyletoposide + sulfate
-
-
-
?
3.1.6.1
4-acetylphenyl sulfate + H2O
-
135518
Streptomyces griseorubiginosus
4-acetylphenol + sulfate
-
-
-
?
3.1.6.1
4-acetylphenyl sulfate + H2O
-
135518
Streptomyces griseorubiginosus S980-14
4-acetylphenol + sulfate
-
-
-
?
3.1.6.1
4-methylumbelliferyl sulfate + H2O
-
135518
Streptomyces griseorubiginosus
4-methylumbelliferone + sulfate
-
-
-
?
3.1.6.1
4-methylumbelliferyl sulfate + H2O
-
135518
Streptomyces griseorubiginosus S980-14
4-methylumbelliferone + sulfate
-
-
-
?
3.1.6.1
4-nitrocatechol sulfate + H2O
-
135518
Streptomyces griseorubiginosus
4-nitrocatechol + sulfate
-
-
-
?
3.1.6.1
4-nitrocatechol sulfate + H2O
-
135518
Streptomyces griseorubiginosus S980-14
4-nitrocatechol + sulfate
-
-
-
?
3.1.6.1
4-nitrophenyl sulfate + H2O
-
135518
Streptomyces griseorubiginosus
4-nitrophenol + sulfate
-
-
-
?
3.1.6.1
4-nitrophenyl sulfate + H2O
-
135518
Streptomyces griseorubiginosus S980-14
4-nitrophenol + sulfate
-
-
-
?
3.1.6.1
androsterone sulfate + H2O
-
135518
Streptomyces griseorubiginosus
androsterone + sulfate
-
-
-
?
3.1.6.1
chondroitin sulfate C + H2O
-
135518
Streptomyces griseorubiginosus
chondroitin + sulfate
-
-
-
?
3.1.6.1
estrone 3-sulfate + H2O
-
135518
Streptomyces griseorubiginosus
sulfate + estrone
-
-
-
?
3.1.6.1
estrone 3-sulfate + H2O
-
135518
Streptomyces griseorubiginosus S980-14
sulfate + estrone
-
-
-
?
3.1.6.1
etoposide 4'-sulfate + H2O
-
135518
Streptomyces griseorubiginosus
etoposide + sulfate
-
-
-
?
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.1.6.1
37
-
-
Streptomyces griseorubiginosus
Temperature Range [°C] (protein specific)
EC Number
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
3.1.6.1
4
50
4°C: about 40% of maximal activity, 50°C: about 45% of maximal activity
Streptomyces griseorubiginosus
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
3.1.6.1
40
-
30 min, stable below
Streptomyces griseorubiginosus
3.1.6.1
50
-
30 min, about 50% loss of activity
Streptomyces griseorubiginosus
3.1.6.1
70
-
30 min, complete loss of activity
Streptomyces griseorubiginosus
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.6.1
8.5
-
-
Streptomyces griseorubiginosus
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
3.1.6.1
7.4
10
pH 7.4: about 35% of maximal activity, 10.0: about 50% of maximal activity
Streptomyces griseorubiginosus
pH Stability (protein specific)
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
3.1.6.1
8
9.5
37°C, 30 min, most stable
Streptomyces griseorubiginosus