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On the physical properties and mechanism of action of arylsulfate sulfohydrolase II from Aspergillus oryzae

Sampson, E.J.; Vergara, E.V.; Fedor, J.M.; Funk, M.O.; Benkovic, S.J.; Arch. Biochem. Biophys. 169, 372-383 (1975)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.6.1
4-nitrophenyl phosphate
hydrolysis of 4-nitrophenyl sulfate
Aspergillus oryzae
3.1.6.1
Estrone 3-sulfate
-
Aspergillus oryzae
3.1.6.1
N-benzyloxycarbonyl-L-Phe-L-Tyr-O-sulfate methyl ester
hydrolysis of 4-nitrophenyl sulfate
Aspergillus oryzae
3.1.6.1
Phenolphthalein disulfate
hydrolysis of 4-nitrophenyl sulfate
Aspergillus oryzae
3.1.6.1
SO42-
competitive
Aspergillus oryzae
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.1.6.1
0.344
-
Estrone 3-sulfate
-
Aspergillus oryzae
3.1.6.1
1.05
-
N-benzyloxycarbonyl-L-Phe-L-Tyr-O-sulfate
-
Aspergillus oryzae
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.1.6.1
40000
-
2 * 40000, SDS-PAGE
Aspergillus oryzae
3.1.6.1
94900
-
sedimentation equilibrium
Aspergillus oryzae
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.1.6.1
Aspergillus oryzae
-
-
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.6.1
2-carboxyphenyl sulfate + H2O
-
135512
Aspergillus oryzae
2-carboxyphenol + sulfate
-
-
-
?
3.1.6.1
2-chloro-4-nitrophenyl sulfate + H2O
-
135512
Aspergillus oryzae
sulfate + 2-chloro-4-nitrophenol
-
-
-
?
3.1.6.1
2-nitrophenyl sulfate + H2O
-
135512
Aspergillus oryzae
2-nitrophenol + sulfate
-
-
-
?
3.1.6.1
4-nitrophenyl sulfate + H2O
-
135512
Aspergillus oryzae
4-nitrophenol + sulfate
-
-
-
?
3.1.6.1
benzyloxycarbonyl-L-Phe-L-Tyr-O-sulfate methyl ester + H2O
-
135512
Aspergillus oryzae
benzyloxycarbonyl-L-Phe-L-Tyr + methylsulfate
-
-
-
?
3.1.6.1
estrone 3-sulfate + H2O
-
135512
Aspergillus oryzae
sulfate + estrone
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
3.1.6.1
dimer
2 * 40000, SDS-PAGE
Aspergillus oryzae
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.6.1
4-nitrophenyl phosphate
hydrolysis of 4-nitrophenyl sulfate
Aspergillus oryzae
3.1.6.1
Estrone 3-sulfate
-
Aspergillus oryzae
3.1.6.1
N-benzyloxycarbonyl-L-Phe-L-Tyr-O-sulfate methyl ester
hydrolysis of 4-nitrophenyl sulfate
Aspergillus oryzae
3.1.6.1
Phenolphthalein disulfate
hydrolysis of 4-nitrophenyl sulfate
Aspergillus oryzae
3.1.6.1
SO42-
competitive
Aspergillus oryzae
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.1.6.1
0.344
-
Estrone 3-sulfate
-
Aspergillus oryzae
3.1.6.1
1.05
-
N-benzyloxycarbonyl-L-Phe-L-Tyr-O-sulfate
-
Aspergillus oryzae
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.1.6.1
40000
-
2 * 40000, SDS-PAGE
Aspergillus oryzae
3.1.6.1
94900
-
sedimentation equilibrium
Aspergillus oryzae
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.6.1
2-carboxyphenyl sulfate + H2O
-
135512
Aspergillus oryzae
2-carboxyphenol + sulfate
-
-
-
?
3.1.6.1
2-chloro-4-nitrophenyl sulfate + H2O
-
135512
Aspergillus oryzae
sulfate + 2-chloro-4-nitrophenol
-
-
-
?
3.1.6.1
2-nitrophenyl sulfate + H2O
-
135512
Aspergillus oryzae
2-nitrophenol + sulfate
-
-
-
?
3.1.6.1
4-nitrophenyl sulfate + H2O
-
135512
Aspergillus oryzae
4-nitrophenol + sulfate
-
-
-
?
3.1.6.1
benzyloxycarbonyl-L-Phe-L-Tyr-O-sulfate methyl ester + H2O
-
135512
Aspergillus oryzae
benzyloxycarbonyl-L-Phe-L-Tyr + methylsulfate
-
-
-
?
3.1.6.1
estrone 3-sulfate + H2O
-
135512
Aspergillus oryzae
sulfate + estrone
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
3.1.6.1
dimer
2 * 40000, SDS-PAGE
Aspergillus oryzae