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Literature summary extracted from

  • Varki, A.; Kornfeld, S.
    Purification and characterization of rat liver alpha-N-acetylglucosaminyl phosphodiesterase (1981), J. Biol. Chem., 256, 9937-9943.
    View publication on PubMed

General Stability

EC Number General Stability Organism
3.1.4.45 loss of activity by repeated freezing and thawing, or dialysis Rattus sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.4.45 mannose-6-phosphate
-
 Rattus sp.
3.1.4.45 N-acetylglucosamine
-
 Rattus sp.
3.1.4.45 N-acetylglucosamine phosphate
-
 Rattus sp.
3.1.4.45 PO43-
-
 Rattus sp.
3.1.4.45 UDP-GlcNAc
-
 Rattus sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.4.45 0.19
-
 methyl 6-O-(N-acetyl-alpha-D-glucosaminyl)phospho-alpha-D-mannose
-
 Rattus sp.
3.1.4.45 1.3
-
 UDP-N-acetylglucosamine
-
 Rattus sp.
3.1.4.45 1.6
-
 N-acetylglucosamine 1-phosphate
-
 Rattus sp.

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.4.45 Golgi membrane cis part Rattus sp. 139
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.4.45 additional information unaffected by divalent cation Rattus sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.4.45 glycoprotein N-acetyl-D-glucosaminyl-phospho-D-mannose + H2O Rattus sp.
-
 N-acetyl-D-glucosamine + glycoprotein phospho-D-mannose
-
 ?
3.1.4.45 additional information Rattus sp. acts on a variety of compounds with N-acetyl-D-glucosamine alpha-linked to phosphate group, including biosynthetic intermediates of the high mannose oligosaccharide components of some lysosomal enzymes ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.4.45 Rattus sp.
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.4.45
-
 Rattus sp.

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Storage Stability

EC Number Storage Stability Organism
3.1.4.45 27% loss of activity after 120 days at -80°C Rattus sp.
3.1.4.45 50% loss of activity after 120 days at 4°C Rattus sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.4.45 glycoprotein N-acetyl-D-glucosaminyl-phospho-D-mannose + H2O
-
 Rattus sp. N-acetyl-D-glucosamine + glycoprotein phospho-D-mannose
-
 ?
3.1.4.45 methyl 6-O-(N-acetyl-alpha-D-glucosaminyl)phospho-alpha-D-mannose + H2O
-
 Rattus sp. N-acetyl-D-glucosamine + methyl alpha-D-mannose 6-phosphate
-
 ?
3.1.4.45 additional information acts on a variety of compounds with N-acetyl-D-glucosamine alpha-linked to phosphate group, including biosynthetic intermediates of the high mannose oligosaccharide components of some lysosomal enzymes Rattus sp. ?
-
 ?
3.1.4.45 N-acetyl-alpha-D-glucosamine 1-phosphate
-
 Rattus sp. N-acetyl-D-glucosamine + phosphate
-
 ?
3.1.4.45 UDP-N-acetyl-alpha-D-glucosamine + H2O
-
 Rattus sp. N-acetyl-D-glucosamine + UDP
-
 ?
3.1.4.45 UDP-N-acetyl-D-glucosamine + H2O
-
 Rattus sp. UDP + N-acetyl-D-glucosamine
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.4.45 6 8
-
 Rattus sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.4.45 4 10
-
 Rattus sp.