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Literature summary extracted from
- Properties and subcellular localization of CMP-N-acetylneuraminic acid hydrolase of calf kidney (1976), Biochim. Biophys. Acta, 444, 816-834.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.4.40 | Triton X-100 | increase of enzyme activity | Bos taurus |  |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.4.40 | 2-mercaptoethanol | - |
Bos taurus | |
| 3.1.4.40 | CMP | 40% inhibition at low CMP-N-acylneuraminate substrate concentrations | Bos taurus | |
| 3.1.4.40 | cytidine | 40% inhibition at low CMP-N-acylneuraminate substrate concentrations | Bos taurus | |
| 3.1.4.40 | EDTA | total loss of activity at 1 mM | Bos taurus | |
| 3.1.4.40 | Mn2+ | slight inhibition at lower concentrations | Bos taurus | |
| 3.1.4.40 | UDP-N-acetylglucosamine | - |
Bos taurus | |
| 3.1.4.40 | UDPgalactose | - |
Bos taurus | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.1.4.40 | 0.47 | - |
CMP-N-acylneuraminate | - |
Bos taurus | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.1.4.40 | microsome | - |
Bos taurus | - |
- |
| 3.1.4.40 | plasma membrane | activity in subfractions | Bos taurus | 5886 | - |
| 3.1.4.40 | plasma membrane | of kidney | Bos taurus | 5886 | - |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.4.40 | Ca2+ | stimulation | Bos taurus | |
| 3.1.4.40 | divalent cations | required, monovalent metal ions without or little stimulatory effect | Bos taurus | |
| 3.1.4.40 | Mg2+ | stimulation | Bos taurus | |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.4.40 | CMP-N-acylneuraminate + H2O | Bos taurus | - |
? | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.1.4.40 | Bos taurus | - |
calf | - |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.1.4.40 | kidney | cortex | Bos taurus | - |
| 3.1.4.40 | kidney | medulla | Bos taurus | - |
| 3.1.4.40 | kidney | papilla | Bos taurus | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.1.4.40 | additional information | - |
- |
Bos taurus |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 3.1.4.40 | -20°C, for at least 5 months, no loss of activity | Bos taurus |
| 3.1.4.40 | 0-4°C, for at several days, no loss of activity | Bos taurus |
| 3.1.4.40 | repeated freezing and thawing, no influence of enzyme activity | Bos taurus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.4.40 | CMP-N-acylneuraminate + H2O | - |
Bos taurus | CMP + N-acylneuraminate | - |
? | |
| 3.1.4.40 | CMP-N-acylneuraminate + H2O | - |
Bos taurus | ? | - |
? | |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.1.4.40 | 45 | - |
- |
Bos taurus |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.4.40 | 8.8 | 9 | - |
Bos taurus |
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Release 2023.1 (January 2023)
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