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Literature summary extracted from
- Purification and characterisation of rape seed phospholipase D (1999), Plant Physiol. Biochem., 37, 531-537.
No PubMed abstract available
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.4.4 | SDS | - |
Brassica napus |  |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.1.4.4 | 1 | 4 | phosphatidylcholine | depends on SDS | Brassica napus | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.4.4 | Ca2+ | 20-100 mM required for maximal activity in plants | Brassica napus | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.1.4.4 | 90000 | 105000 | gel filtration, SDS-PAGE | Brassica napus |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.4.4 | phospholipid + H2O | Brassica napus | phosphoric ester hydrolysis | phosphatidic acid + alcohol | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.1.4.4 | Brassica napus | - |
Winter rape | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.1.4.4 | complete to homogeneity | Brassica napus |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.1.4.4 | seed | - |
Brassica napus | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.4.4 | phosphatidylcholine + H2O | - |
Brassica napus | 1,2-diacylglycerophosphate + choline | - |
? | |
| 3.1.4.4 | phosphatidylethanolamine + H2O | - |
Brassica napus | 1,2-diacylglycerophosphate + ethanolamine | - |
? | |
| 3.1.4.4 | phospholipid + H2O | phosphoric ester hydrolysis | Brassica napus | phosphatidic acid + alcohol | - |
? | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.4.4 | 5.5 | 6 | - |
Brassica napus |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.4.4 | 4.5 | 7 | - |
Brassica napus |
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Release 2023.1 (January 2023)
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