BRENDA - Enzyme Database

Purification and characterization of phosphatidylcholine phospholipase D from pig lung

Okamura, S.; Yamashita, S.; J. Biol. Chem. 269, 31207-31213 (1994)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
3.1.4.4
arachidonic acid
only in presence of Mg2+
Sus scrofa
3.1.4.4
linoleic acid
only in presence of Mg2+
Sus scrofa
3.1.4.4
lysophosphatidic acid
-
Sus scrofa
3.1.4.4
oleic acid
only in presence of Mg2+
Sus scrofa
3.1.4.4
phosphatidylinositol 4,5-bisphosphate
-
Sus scrofa
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.4.4
lysophosphatidylcholine
-
Sus scrofa
3.1.4.4
N-ethylmaleimide
-
Sus scrofa
3.1.4.4
palmitic acid
-
Sus scrofa
3.1.4.4
phosphatidic acid
-
Sus scrofa
3.1.4.4
phosphatidylethanolamine
-
Sus scrofa
3.1.4.4
phosphatidylinositol
-
Sus scrofa
3.1.4.4
stearic acid
-
Sus scrofa
3.1.4.4
sucrose monolaurate
-
Sus scrofa
3.1.4.4
Triton X-100
-
Sus scrofa
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.1.4.4
0.8
-
phosphatidylcholine
-
Sus scrofa
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.1.4.4
membrane
-
Sus scrofa
16020
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
3.1.4.4
Ca2+
micromolar levels for maximal activity
Sus scrofa
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.1.4.4
190000
-
gel filtration, SDS-PAGE
Sus scrofa
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.1.4.4
phospholipid + alcohol
Sus scrofa
transphosphaditylation
phospholipid + alcohol
-
-
?
3.1.4.4
phospholipid + H2O
Sus scrofa
phosphoric ester hydrolysis
phosphatidic acid + alcohol
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.1.4.4
Sus scrofa
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
3.1.4.4
complete to homogeneity
Sus scrofa
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.4.4
lung
-
Sus scrofa
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
3.1.4.4
3.67
-
-
Sus scrofa
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.4.4
phosphatidylcholine + ethanol
-
135449
Sus scrofa
phosphatidylethanol + choline
-
-
-
?
3.1.4.4
phosphatidylcholine + ethanolamine
-
135449
Sus scrofa
phosphatidylethanolamine + choline
-
-
-
?
3.1.4.4
phosphatidylcholine + H2O
-
135449
Sus scrofa
1,2-diacylglycerophosphate + choline
-
-
-
?
3.1.4.4
phosphatidylinositol + H2O
-
135449
Sus scrofa
1,2-diacylglycerophosphate + inositol
-
-
-
?
3.1.4.4
phospholipid + alcohol
transphosphaditylation
135449
Sus scrofa
phospholipid + alcohol
-
-
-
?
3.1.4.4
phospholipid + H2O
phosphoric ester hydrolysis
135449
Sus scrofa
phosphatidic acid + alcohol
-
-
-
?
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.4.4
6.6
-
-
Sus scrofa
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
3.1.4.4
arachidonic acid
only in presence of Mg2+
Sus scrofa
3.1.4.4
linoleic acid
only in presence of Mg2+
Sus scrofa
3.1.4.4
lysophosphatidic acid
-
Sus scrofa
3.1.4.4
oleic acid
only in presence of Mg2+
Sus scrofa
3.1.4.4
phosphatidylinositol 4,5-bisphosphate
-
Sus scrofa
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.4.4
lysophosphatidylcholine
-
Sus scrofa
3.1.4.4
N-ethylmaleimide
-
Sus scrofa
3.1.4.4
palmitic acid
-
Sus scrofa
3.1.4.4
phosphatidic acid
-
Sus scrofa
3.1.4.4
phosphatidylethanolamine
-
Sus scrofa
3.1.4.4
phosphatidylinositol
-
Sus scrofa
3.1.4.4
stearic acid
-
Sus scrofa
3.1.4.4
sucrose monolaurate
-
Sus scrofa
3.1.4.4
Triton X-100
-
Sus scrofa
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.1.4.4
0.8
-
phosphatidylcholine
-
Sus scrofa
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.1.4.4
membrane
-
Sus scrofa
16020
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
3.1.4.4
Ca2+
micromolar levels for maximal activity
Sus scrofa
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.1.4.4
190000
-
gel filtration, SDS-PAGE
Sus scrofa
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.1.4.4
phospholipid + alcohol
Sus scrofa
transphosphaditylation
phospholipid + alcohol
-
-
?
3.1.4.4
phospholipid + H2O
Sus scrofa
phosphoric ester hydrolysis
phosphatidic acid + alcohol
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.1.4.4
complete to homogeneity
Sus scrofa
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.4.4
lung
-
Sus scrofa
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
3.1.4.4
3.67
-
-
Sus scrofa
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.4.4
phosphatidylcholine + ethanol
-
135449
Sus scrofa
phosphatidylethanol + choline
-
-
-
?
3.1.4.4
phosphatidylcholine + ethanolamine
-
135449
Sus scrofa
phosphatidylethanolamine + choline
-
-
-
?
3.1.4.4
phosphatidylcholine + H2O
-
135449
Sus scrofa
1,2-diacylglycerophosphate + choline
-
-
-
?
3.1.4.4
phosphatidylinositol + H2O
-
135449
Sus scrofa
1,2-diacylglycerophosphate + inositol
-
-
-
?
3.1.4.4
phospholipid + alcohol
transphosphaditylation
135449
Sus scrofa
phospholipid + alcohol
-
-
-
?
3.1.4.4
phospholipid + H2O
phosphoric ester hydrolysis
135449
Sus scrofa
phosphatidic acid + alcohol
-
-
-
?
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.4.4
6.6
-
-
Sus scrofa