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Literature summary extracted from

  • Heller, M.; Mozes, N.; Peri, I.; Maes, E.
    Phospholipase D from peanut seeds. Final purification and some properties of the enzyme (1974), Biochim. Biophys. Acta, 369, 397-410.
No PubMed abstract available

General Stability

EC Number General Stability Organism
3.1.4.4 enzyme dilutions retain activity at neutral pH Arachis hypogaea

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.4.4 Ca2+ 20-100 mM required for maximal activity in plants Arachis hypogaea

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.4.4 22000
-
 sedimentation Arachis hypogaea
3.1.4.4 46000 50000 gel filtration in the presence of reducing agents, SDS-PAGE Arachis hypogaea
3.1.4.4 50000
-
 ? * 50000, ? * 22000 time-dependent dissociation into smaller subunits possible, gel filtration in the presence of reducing agents Arachis hypogaea
3.1.4.4 200000
-
 time-dependent dissociation into smaller subunits possible, gel filtration, SDS-PAGE Arachis hypogaea

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.4.4 phospholipid + H2O Arachis hypogaea phosphoric ester hydrolysis phosphatidic acid + alcohol
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.4.4 Arachis hypogaea
-
 peanut
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.4.4 complete to homogeneity Arachis hypogaea

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.4.4 seed
-
 Arachis hypogaea
-

Storage Stability

EC Number Storage Stability Organism
3.1.4.4 -20°C, neutral pH, 0.5 M glycerol, 20 days, no loss of activity Arachis hypogaea
3.1.4.4 -20°C, neutral pH, few days, 95% loss of activity Arachis hypogaea
3.1.4.4 20°C, neutral pH, 15 days, no loss of activity Arachis hypogaea
3.1.4.4 4°C, neutral pH, 50 days, no loss of activity Arachis hypogaea

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.4.4 phospholipid + H2O phosphoric ester hydrolysis Arachis hypogaea phosphatidic acid + alcohol
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.4.4 polymer ? * 50000, ? * 22000 time-dependent dissociation into smaller subunits possible, gel filtration in the presence of reducing agents Arachis hypogaea

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.4.4 5.6
-
-
 Arachis hypogaea

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.1.4.4 4.5
-
 irreversible loss of activity at acid pH Arachis hypogaea