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Literature summary extracted from

  • Kokusho, Y.; Kato, S.; Machida, H.; Iwasaki, S.
    Purification and properties of phospholipase D from Actinomadura sp.strain no.362 (1987), Agric. Biol. Chem., 51, 2515-2524.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.4.4 cholic acid
-
 Actinomadura sp.
3.1.4.4 ethyl ether stimulates Actinomadura sp.
3.1.4.4 sodium deoxycholate
-
 Actinomadura sp.
3.1.4.4 Triton X-100 substrate-dependent activation Actinomadura sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.4.4 Ag2+ in the presence of Triton X-100 Actinomadura sp.
3.1.4.4 Al3+ stimulates in the absence of Triton X-100 Actinomadura sp.
3.1.4.4 Cetylpyridinium chloride
-
 Actinomadura sp.
3.1.4.4 cetyltrimethylammonium bromide
-
 Actinomadura sp.
3.1.4.4 Fe2+ stimulates in the absence of Triton X-100 Actinomadura sp.
3.1.4.4 Fe3+ stimulates in the absence of Triton X-100 Actinomadura sp.
3.1.4.4 Hg2+ in the presence of Triton X-100 Actinomadura sp.
3.1.4.4 Mn2+ stimulates in the absence of Triton X-100 Actinomadura sp.
3.1.4.4 SDS
-
 Actinomadura sp.
3.1.4.4 Sn2+ stimulates in the absence of Triton X-100 Actinomadura sp.
3.1.4.4 Triton X-100 substrate-dependent inhibition Actinomadura sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.4.4 0.7
-
 phosphatidylcholine in the presence of Triton X-100 Actinomadura sp.
3.1.4.4 1.07
-
 lysophosphatidylcholine in the presence of Triton X-100 Actinomadura sp.

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.4.4 extracellular
-
 Actinomadura sp.
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.4.4 Al3+ stimulates in the absence of Triton X-100 Actinomadura sp.
3.1.4.4 Ca2+
-
 Actinomadura sp.
3.1.4.4 Fe2+ stimulates in the absence of Triton X-100 Actinomadura sp.
3.1.4.4 Fe3+ stimulates in the absence of Triton X-100 Actinomadura sp.
3.1.4.4 Mn2+ stimulates in the absence of Triton X-100 Actinomadura sp.
3.1.4.4 Sn2+ stimulates in the absence of Triton X-100 Actinomadura sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.4.4 50000 60000 gel filtration, SDS-PAGE Actinomadura sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.4.4 phospholipid + alcohol Actinomadura sp. transphosphaditylation phospholipid + alcohol
-
 ?
3.1.4.4 phospholipid + H2O Actinomadura sp. phosphoric ester hydrolysis phosphatidic acid + alcohol
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.4.4 Actinomadura sp.
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.4.4 to homogeneity Actinomadura sp.

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.4.4 culture supernatant
-
 Actinomadura sp.
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.4.4 688
-
-
 Actinomadura sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.4.4 lysophosphatidylcholine + H2O
-
 Actinomadura sp. monoacylglycerophosphate + choline
-
 ?
3.1.4.4 phosphatidylcholine + ethanolamine
-
 Actinomadura sp. phosphatidylethanolamine + choline
-
 ?
3.1.4.4 phosphatidylcholine + H2O
-
 Actinomadura sp. 1,2-diacylglycerophosphate + choline
-
 ?
3.1.4.4 phosphatidylethanolamine + H2O
-
 Actinomadura sp. 1,2-diacylglycerophosphate + ethanolamine
-
 ?
3.1.4.4 phospholipid + alcohol transphosphaditylation Actinomadura sp. phospholipid + alcohol
-
 ?
3.1.4.4 phospholipid + H2O phosphoric ester hydrolysis Actinomadura sp. phosphatidic acid + alcohol
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.4.4 50
-
 in the presence of Triton X-100 Actinomadura sp.
3.1.4.4 60 70 in the absence of Triton X-100 Actinomadura sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.4.4 30
-
 at pH 7.2 for 30 min Actinomadura sp.
3.1.4.4 30
-
 irreversible loss of activity at higher temperature Actinomadura sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.4.4 5.5 7 Triton X-100 dependent Actinomadura sp.

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.1.4.4 4 8 2 h at 25°C Actinomadura sp.