Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Seki, T.; Fukuda, S.
    Purification and some properties of 2',3'-cyclic phosphodiesterase from the cell-free extract of Bacillus subtilis var. amyloliquefacus (1981), J. Gen. Appl. Microbiol., 27, 487-498.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.4.37 Co2+
-
 Bacillus subtilis
3.1.4.37 Cu2+
-
 Bacillus subtilis
3.1.4.37 EDTA
-
 Bacillus subtilis
3.1.4.37 Hg2+
-
 Bacillus subtilis
3.1.4.37 Zn2+
-
 Bacillus subtilis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.4.37 0.046
-
 3'-AMP
-
 Bacillus subtilis
3.1.4.37 0.16
-
 bis-p-nitrophenyl phosphate
-
 Bacillus subtilis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.4.37 Co2+ activation of activity against bis-p-nitrophenyl phosphate Bacillus subtilis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.4.37 74000
-
 x * 74000, SDS-PAGE Bacillus subtilis

Organism

EC Number Organism UniProt Comment Textmining
3.1.4.37 Bacillus subtilis
-
 var. amyloliquefacus
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.4.37
-
 Bacillus subtilis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.4.37 additional information
-
-
 Bacillus subtilis

Storage Stability

EC Number Storage Stability Organism
3.1.4.37 0-4°C, 5 mM Tris-HCl buffer, pH 7.6, stable for 2 months Bacillus subtilis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.4.37 3'-AMP + H2O
-
 Bacillus subtilis ?
-
 ?
3.1.4.37 bis-p nitrophenyl phosphate + H2O
-
 Bacillus subtilis ?
-
 ?
3.1.4.37 additional information
-
 Bacillus subtilis ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.4.37 ? x * 74000, SDS-PAGE Bacillus subtilis

Synonyms

EC Number Synonyms Comment Organism
3.1.4.37 2',3'-cyclic phosphodiesterase
-
 Bacillus subtilis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.4.37 30
-
-
 Bacillus subtilis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.4.37 50 70 inactivation Bacillus subtilis
3.1.4.37 100
-
 80% loss of activity, and completely eliminated after 20 min, Co2+ protects against heat inactivation Bacillus subtilis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.4.37 5
-
 activity against bis-p-nitrophenyl phosphate Bacillus subtilis
3.1.4.37 6.7
-
-
 Bacillus subtilis