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Literature summary extracted from

  • Hirai, K.; Matsuda, Y.; Nakagawa, H.
    Purification and characterization of GMP synthetase from Yoshida sarcoma ascites cells (1987), J. Biochem., 102, 893-902.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
6.3.5.2 reduced sulfhydryl compounds e.g. dithiothreitol, required Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.3.5.2 AMP
-
 Rattus norvegicus
6.3.5.2 cAMP
-
 Rattus norvegicus
6.3.5.2 cGMP
-
 Rattus norvegicus
6.3.5.2 CMP
-
 Rattus norvegicus
6.3.5.2 GMP
-
 Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.3.5.2 0.0046
-
 XMP
-
 Rattus norvegicus
6.3.5.2 0.12
-
 ATP
-
 Rattus norvegicus
6.3.5.2 0.3
-
 Gln
-
 Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
6.3.5.2 cytosol Yoshida sarcoma ascites cells Rattus norvegicus 5829
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.3.5.2 Mg2+ high activity only in presence of Mg2+ Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.3.5.2 78000
-
 gel filtration Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
6.3.5.2 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.3.5.2 Yoshida sarcoma ascites cells Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
6.3.5.2 adipose tissue
-
 Rattus norvegicus
-
6.3.5.2 brain
-
 Rattus norvegicus
-
6.3.5.2 heart
-
 Rattus norvegicus
-
6.3.5.2 intestine
-
 Rattus norvegicus
-
6.3.5.2 kidney
-
 Rattus norvegicus
-
6.3.5.2 liver
-
 Rattus norvegicus
-
6.3.5.2 lung
-
 Rattus norvegicus
-
6.3.5.2 ovary
-
 Rattus norvegicus
-
6.3.5.2 skeletal muscle
-
 Rattus norvegicus
-
6.3.5.2 spleen
-
 Rattus norvegicus
-
6.3.5.2 testis
-
 Rattus norvegicus
-
6.3.5.2 thymus
-
 Rattus norvegicus
-
6.3.5.2 Yoshida ascites sarcoma cell
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.3.5.2 0.22
-
-
 Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.5.2 ATP + XMP + Gln maximal activity with NH4+ is 80% of that obtained with Gln Rattus norvegicus AMP + diphosphate + GMP + Glu
-
 ?
6.3.5.2 ATP + XMP + Gln higher Km value for NH4+ compared with Gln Rattus norvegicus AMP + diphosphate + GMP + Glu
-
 ?
6.3.5.2 ATP + XMP + NH4+ maximal activity with NH4+ is 80% of that obtained with Gln Rattus norvegicus AMP + diphosphate + GMP
-
 ?
6.3.5.2 ATP + XMP + NH4+ higher Km value for NH4+ compared with Gln Rattus norvegicus AMP + diphosphate + GMP
-
 ?

Subunits

EC Number Subunits Comment Organism
6.3.5.2 monomer 1 * 78000, SDS-PAGE Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.3.5.2 7.8 8
-
 Rattus norvegicus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
6.3.5.2 6.5 9 more than 50% of maximal activity at pH 6.5 and pH 9.0 Rattus norvegicus