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Literature summary extracted from

  • Nishizawa, Y.; Kurihara, T.; Takahshi, T.
    Spectrophotometric assay, solubilization and purification of brain 2':3'-cyclic nucleotide 3-phosphodiesterase (1980), Biochem. J., 191, 71-82.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.4.37 hexadecyltrimethylammonium bromide
-
 Bos taurus
3.1.4.37 additional information solublization of the enzyme by elastase or trypsin Bos taurus

General Stability

EC Number General Stability Organism
3.1.4.37 dilution, inactivates Bos taurus
3.1.4.37 freezing and thawing, inactivates Bos taurus
3.1.4.37 serum albumin, 2'-AMP and NaCl, stabilizes Bos taurus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.4.37 Elastase little inactivation Bos taurus
3.1.4.37 heparin
-
 Bos taurus
3.1.4.37 Trypsin inactivation Bos taurus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.4.37 30000
-
 1 * 30000, SDS-PAGE Bos taurus
3.1.4.37 31000
-
 gel filtration Bos taurus

Organism

EC Number Organism UniProt Comment Textmining
3.1.4.37 Bos taurus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.4.37 partial, by delipidation, solubilization, gel chromatography, resolubilization, ion-exchange chromatography and affinity chromatography Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.4.37 brain
-
 Bos taurus
-
3.1.4.37 centrum semiovale
-
 Bos taurus
-
3.1.4.37 cerebral white matter
-
 Bos taurus
-
3.1.4.37 corpus callosum
-
 Bos taurus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.4.37 2680
-
-
 Bos taurus

Storage Stability

EC Number Storage Stability Organism
3.1.4.37 4°C, 6 months, more than 90% of activity retained Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.4.37 2',3'-cAMP + H2O
-
 Bos taurus 2'-AMP
-
 ?
3.1.4.37 nucleoside 2',3'-cyclic phosphate + H2O
-
 Bos taurus nucleoside 2'-phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.4.37 ? 27000-31000, SDS-PAGE Bos taurus
3.1.4.37 monomer 1 * 30000, SDS-PAGE Bos taurus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.4.37 6.6
-
-
 Bos taurus