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Literature summary extracted from

  • Tyc, K.; Kellenberger, C.; Filipowicz, W.
    Purification and characterization of wheat germ 2,3-cyclic nucleotide 3-phosphodiesterase (1987), J. Biol. Chem., 262, 12994-1300.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.4.37 bovine serum albumin
-
 Triticum aestivum
3.1.4.37 Triton X-100
-
 Triticum aestivum

General Stability

EC Number General Stability Organism
3.1.4.37 freezing and thawing, not affected by several cycles Triticum aestivum

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.4.37 Ca2+ 74% inhibition at 0.5 mM Triticum aestivum
3.1.4.37 Cu2+ 95% inhibition at 0.5 mM Triticum aestivum
3.1.4.37 Fe2+ 33% inhibition at 0.5 mM Triticum aestivum
3.1.4.37 Fe3+ 66% inhibition at 0.5 mM Triticum aestivum
3.1.4.37 NaCl
-
 Triticum aestivum
3.1.4.37 PO43-
-
 Triticum aestivum
3.1.4.37 Zn2+ 82% inhibition at 0.5 mM Triticum aestivum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.4.37 additional information
-
 additional information
-
 Triticum aestivum
3.1.4.37 9.2
-
 2',3'-cGMP
-
 Triticum aestivum
3.1.4.37 13.1
-
 2',3'-cAMP
-
 Triticum aestivum
3.1.4.37 25.2
-
 2',3'-cCMP
-
 Triticum aestivum
3.1.4.37 25.3
-
 2',3'-cUMP
-
 Triticum aestivum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.4.37 Mn2+ slight stimulation Triticum aestivum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.4.37 23000
-
 gel filtration Triticum aestivum
3.1.4.37 24000
-
 x * 24000, SDS-PAGE Triticum aestivum

Organism

EC Number Organism UniProt Comment Textmining
3.1.4.37 Triticum aestivum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.4.37 16000fold to near homogeneity Triticum aestivum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.4.37 germ
-
 Triticum aestivum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.4.37 280
-
 2',3'-cGMP Triticum aestivum
3.1.4.37 600
-
 2',3'-cUMP Triticum aestivum
3.1.4.37 2090
-
 2',3'-cAMP Triticum aestivum
3.1.4.37 2140
-
 2',3'-cCMP Triticum aestivum

Storage Stability

EC Number Storage Stability Organism
3.1.4.37 -70°C, stable for at least 5 months Triticum aestivum
3.1.4.37 4°C, Tris-acetate buffer, 0.5 mM dithiothreitol, pH 7.6, 10% glycerol, protease inhibitor mix, 3 days Triticum aestivum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.4.37 2',3'-cAMP + H2O
-
 Triticum aestivum 2'-AMP
-
 ?
3.1.4.37 2',3'-cCMP + H2O
-
 Triticum aestivum 2'-CMP
-
 ?
3.1.4.37 2',3'-cGMP + H2O
-
 Triticum aestivum 2'-GMP
-
 ?
3.1.4.37 2',3'-cUMP + H2O
-
 Triticum aestivum 2'-UMP
-
 ?
3.1.4.37 additional information not: 2',3'-cyclic esters in cyclic phosphate-terminated oligoribonucleotides or in nucleoside 5'-phosphate, 2',3'-cyclic phosphate Triticum aestivum ?
-
 ?
3.1.4.37 nucleoside 2',3'-cyclic phosphate + H2O
-
 Triticum aestivum nucleoside 2'-phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.4.37 ? x * 24000, SDS-PAGE Triticum aestivum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.4.37 55
-
 no loss of activity after 10 min Triticum aestivum
3.1.4.37 65
-
 20% loss of activity after 10 min Triticum aestivum
3.1.4.37 80
-
 82.2% loss of activity after 10 min Triticum aestivum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.4.37 7
-
-
 Triticum aestivum

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.4.37 5 9
-
 Triticum aestivum