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Literature summary extracted from

  • Morishima, I.
    Cylic nucleotide phosphodiesterase in silkworm. Characterization of cyclic GMP phosphodiesterase (1975), Biochim. Biophys. Acta, 410, 310-317.
    View publication on PubMed

General Stability

EC Number General Stability Organism
3.1.4.35 5 mM beta-mercaptoethanol and bovine serum albumin Bombyx mori
3.1.4.35 mercaptoethanol, stabilization Bombyx mori

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.4.35 2',3'-cGMP
-
 Bombyx mori
3.1.4.35 3',5'-cAMP 20% inhibition at 0.1 mM, 50% inhibition at 1 mM Bombyx mori
3.1.4.35 3',5'-cIMP
-
 Bombyx mori
3.1.4.35 ATP slight inhibition at 1 mM Bombyx mori
3.1.4.35 Caffeine
-
 Bombyx mori
3.1.4.35 dibutyryl 3',5'-cAMP
-
 Bombyx mori
3.1.4.35 EDTA completely inhibits at 10 mM Bombyx mori
3.1.4.35 GTP slight inhibition at 1 mM Bombyx mori
3.1.4.35 theophylline
-
 Bombyx mori

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.4.35 0.002
-
 3',5'-cGMP
-
 Bombyx mori

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.4.35 Mg2+ 5 mM activates Bombyx mori
3.1.4.35 Mn2+ 2 mM activates Bombyx mori

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.4.35 260000
-
 gel filtration Bombyx mori

Organism

EC Number Organism UniProt Comment Textmining
3.1.4.35 Bombyx mori
-
 silkworm
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.4.35
-
 Bombyx mori

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.4.35 larva
-
 Bombyx mori
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.4.35 additional information
-
-
 Bombyx mori

Storage Stability

EC Number Storage Stability Organism
3.1.4.35 0°C Bombyx mori

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.4.35 3',5'-cGMP + H2O
-
 Bombyx mori 5'-GMP
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.4.35 8.3
-
 with Tris-acetate as buffer Bombyx mori

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.1.4.35 1
-
 3',5'-cAMP
-
 Bombyx mori